Solution structure of deglycosylated human IgG1 shows the role of CH2 glycans in its conformation

Spiteri V, Doutch J, Rambo R, Gor J, Dalby P, Perkins S
Biophysical Journal (2021 Mar)

doi: 10.1016/j.bpj.2021.02.038 		Submitted to SASBDB: 2021 Feb 5
Published in SASBDB:

SASDKB8 – Immunoglobulin G1 (IgG1) Glycosylated

Immunoglobulin G subclass 1 experimental SAS data
CUSTOM IN-HOUSE model
Sample: Immunoglobulin G subclass 1, 148 kDa Homo sapiens protein
Buffer: 20 mM L-histidine, 138 mM NaCl, and 2.6 mM KCl buffer, pH: 6
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Oct 20
RgGuinier 5.1 nm
Dmax 17.5 nm
VolumePorod 269 nm3

SASDKC8 – Immunoglobulin G1 (IgG1) Deglycosylated

Immunoglobulin G subclass 1 experimental SAS data
CUSTOM IN-HOUSE model
Sample: Immunoglobulin G subclass 1, 148 kDa Homo sapiens protein
Buffer: 20 mM L-histidine, 138 mM NaCl, and 2.6 mM KCl buffer, pH: 6
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Oct 20
RgGuinier 5.2 nm
Dmax 18.4 nm
VolumePorod 271 nm3