Rational thermostabilisation of four-helix bundle dimeric de novo proteins.

Irumagawa S, Kobayashi K, Saito Y, Miyata T, Umetsu M, Kameda T, Arai R
Sci Rep 11(1):7526 (2021 Apr 6)
PMID: 33824364
doi: 10.1038/s41598-021-86952-2 		Submitted to SASBDB: 2021 Feb 19
Published in SASBDB:

SASDKM8 – Rationally optimised WA20 (ROWA) dimer

Rationally optimised WA20 mutant N22A/H86K (ROWA) dimer experimental SAS data
DAMMIN model
Sample: Rationally optimised WA20 mutant N22A/H86K (ROWA) dimer dimer, 25 kDa Artificial protein (de … protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5% glycerol,, pH: 7.5
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2019 Nov 30
RgGuinier 2.5 nm
Dmax 9.9 nm
VolumePorod 30 nm3

SASDKN8 – Rationally optimised WA20 (ROWA) tetramer

Rationally optimised WA20 mutant N22A/H86K (ROWA) tetramer experimental SAS data
DAMMIN model
Sample: Rationally optimised WA20 mutant N22A/H86K (ROWA) tetramer tetramer, 50 kDa Artificial protein (de … protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5% glycerol,, pH: 7.5
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2019 Nov 30
RgGuinier 4.1 nm
Dmax 18.1 nm
VolumePorod 60 nm3

SASDKP8 – Rationally optimised WA20 (ROWA) hexamer

Rationally optimised WA20 mutant N22A/H86K (ROWA) hexamer experimental SAS data
DAMMIN model
Sample: Rationally optimised WA20 mutant N22A/H86K (ROWA) hexamer hexamer, 75 kDa Artificial protein (de … protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5% glycerol,, pH: 7.5
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2019 Nov 30
RgGuinier 5.3 nm
Dmax 24.8 nm
VolumePorod 120 nm3

SASDKQ8 – Rationally optimised SUWA (ROSA) dimer

Rationally optimised SUWA mutant N22E/H86K (ROSA) dimer experimental SAS data
DAMMIN model
Sample: Rationally optimised SUWA mutant N22E/H86K (ROSA) dimer dimer, 25 kDa Artificial protein (de … protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5% glycerol,, pH: 7.5
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2019 Nov 30
RgGuinier 2.5 nm
Dmax 10.6 nm
VolumePorod 30 nm3

SASDKR8 – Rationally optimised SUWA (ROSA) tetramer

Rationally optimised SUWA mutant N22E/H86K (ROSA) tetramer experimental SAS data
DAMMIN model
Sample: Rationally optimised SUWA mutant N22E/H86K (ROSA) tetramer tetramer, 50 kDa Artificial protein (de … protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5% glycerol,, pH: 7.5
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2019 Nov 30
RgGuinier 4.4 nm
Dmax 22.1 nm
VolumePorod 88 nm3