An Integrative Structural Biology Analysis of Von Willebrand Factor Binding and Processing by ADAMTS-13 in Solution.

Del Amo-Maestro L, Sagar A, Pompach P, Goulas T, Scavenius C, Ferrero DS, Castrillo-Briceño M, Taulés M, Enghild JJ, Bernadó P, Gomis-Rüth FX
J Mol Biol 433(13):166954 (2021 Mar 24)

PMID: 33771572 doi: 10.1016/j.jmb.2021.166954

Submitted to SASBDB: 2021 Feb 24 Published in SASBDB: 2021 May 10

SASDKT8 – von Willebrand Factor peptide 3mg/ml Sample: Von Willebrand factor monomer, 11 kDa Homo sapiens protein Buffer: 10 mM HEPES, 150 mM NaCl, pH: 7.4 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Aug 24 RgGuinier 3.1 nm Dmax 14.0 nm VolumePorod 28 nm3

SASDKU8 – von Willebrand Factor peptide 6mg/ml Sample: Von Willebrand factor monomer, 11 kDa Homo sapiens protein Buffer: 10 mM HEPES, 150 mM NaCl, pH: 7.4 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Aug 24 RgGuinier 3.0 nm Dmax 14.9 nm VolumePorod 27 nm3

SASDKV8 – ADAMTS-13 bound to von Willebrand Factor peptide Sample: Von Willebrand factor monomer, 11 kDa Homo sapiens protein A disintegrin and metalloproteinase with thrombospondin motifs 13 monomer, 70 kDa Homo sapiens protein Buffer: 10 mM HEPES, 150 mM NaCl, pH: 7.4 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Aug 24 RgGuinier 4.4 nm Dmax 16.3 nm VolumePorod 170 nm3