Using atomistic solution scattering modelling to elucidate the role of the Fc glycans in human IgG4.

Spiteri VA, Doutch J, Rambo RP, Bhatt JS, Gor J, Dalby PA, Perkins SJ
PLoS One 19(4):e0300964 (2024)
PMID: 38557973
doi: 10.1371/journal.pone.0300964 		Submitted to SASBDB: 2021 Apr 6
Published in SASBDB:

SASDLX2 – Immunoglobulin G4 (IgG4) Deglycosylated

Immunoglobulin G subclass 4 experimental SAS data
CUSTOM IN-HOUSE model
Sample: Immunoglobulin G subclass 4 monomer, 145 kDa Homo sapiens protein
Buffer: 20 mM L-histidine, 138 mM NaCl, and 2.6 mM KCl buffer, pH: 6
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Oct 20
RgGuinier 4.9 nm
Dmax 16.9 nm
VolumePorod 258 nm3

SASDLY2 – Immunoglobulin G4 (IgG4) Glycosylated

Immunoglobulin G subclass 4 experimental SAS data
CUSTOM IN-HOUSE model
Sample: Immunoglobulin G subclass 4 monomer, 145 kDa Homo sapiens protein
Buffer: 20 mM L-histidine, 138 mM NaCl, and 2.6 mM KCl buffer, pH: 6
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Oct 20
RgGuinier 4.9 nm
Dmax 16.3 nm
VolumePorod 256 nm3