Probing solution structure of the pentameric ligand-gated ion channel GLIC by small-angle neutron scattering

Lycksell M, Rovšnik U, Bergh C, Johansen N, Martel A, Porcar L, Arleth L, Howard R, Lindahl E
Proceedings of the National Academy of Sciences 118(37):e2108006118 (2021 Sep 09)

doi: 10.1073/pnas.2108006118

Submitted to SASBDB: 2021 Apr 10 Published in SASBDB: 2021 Aug 23

SASDL33 – Gloeobacter violaceus Ligand-Gated Ion Channel (GLIC) at pH 7.5 measured with paused-flow SEC-SANS Sample: Proton-gated ion channel pentamer, 183 kDa Gloeobacter violaceus (strain … protein Buffer: D2O, 20 mM Tris, 150 mM NaCl, 0.5 mM matched-out deuterated DDM,, pH: 7.5 Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2020 Aug 22 RgGuinier 3.8 nm Dmax 13.5 nm VolumePorod 274 nm3

SASDL43 – Gloeobacter violaceus Ligand-Gated Ion Channel (GLIC) at pH 3.0 measured with paused-flow SEC-SANS Sample: Proton-gated ion channel pentamer, 183 kDa Gloeobacter violaceus (strain … protein Buffer: D2O, 20 mM citrate, 150 mM NaCl, 0.5 mM match-out deuterated DDM, pH: 3 Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2020 Aug 22 RgGuinier 3.8 nm Dmax 12.7 nm VolumePorod 279 nm3

SASDL53 – Gloeobacter violaceus Ligand-Gated Ion Channel (GLIC) at pH 7.5 measured with continuous-flow SEC-SANS Sample: Proton-gated ion channel pentamer, 183 kDa Gloeobacter violaceus (strain … protein Buffer: D2O, 20 mM Tris, 150 mM NaCl, 0.5 mM matched-out deuterated DDM,, pH: 7.5 Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2019 Jun 20 RgGuinier 3.8 nm Dmax 12.0 nm VolumePorod 235 nm3

SASDL63 – Gloeobacter violaceus Ligand-Gated Ion Channel (GLIC) at pH 7.5 measured with cuvette-mode SANS Sample: Proton-gated ion channel pentamer, 183 kDa Gloeobacter violaceus (strain … protein Buffer: D2O, 20 mM Tris, 150 mM NaCl, 0.5 mM matched-out deuterated DDM,, pH: 7.5 Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2019 Jun 21 RgGuinier 4.0 nm Dmax 17.7 nm VolumePorod 225 nm3