Autism associated SHANK3 missense point mutations impact conformational fluctuations and protein turnover at synapses.

Bucher M, Niebling S, Han Y, Molodenskiy D, Nia FH, Kreienkamp HJ, Svergun D, Kim E, Kostyukova AS, Kreutz MR, Mikhaylova M
Elife 10 (2021 May 4)
PMID: 33945465
doi: 10.7554/eLife.66165 		Published in SASBDB:

SASDLJ3 – SH3 and multiple ankyrin repeat domains protein 3 (wild type)

SH3 and multiple ankyrin repeat domains protein 3 experimental SAS data
CORAL model
Sample: SH3 and multiple ankyrin repeat domains protein 3 monomer, 88 kDa Rattus norvegicus protein
Buffer: 100mM NaH2PO4, 100mM NaCl, 0.5mM DTT,, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Jun 3
RgGuinier 4.1 nm
Dmax 14.0 nm
VolumePorod 170 nm3

SASDLK3 – SH3 and multiple ankyrin repeat domains protein 3 with a point mutation (L68P)

SH3 and multiple ankyrin repeat domains protein 3 experimental SAS data
CORAL model
Sample: SH3 and multiple ankyrin repeat domains protein 3 monomer, 88 kDa Rattus norvegicus protein
Buffer: 100mM NaH2PO4, 100mM NaCl, 0.5mM DTT,, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Jun 3
RgGuinier 4.1 nm
Dmax 14.8 nm
VolumePorod 224 nm3

SASDLL3 – SH3 and multiple ankyrin repeat domains protein 3 with a point mutation (R12C)

SH3 and multiple ankyrin repeat domains protein 3 experimental SAS data
CORAL model
Sample: SH3 and multiple ankyrin repeat domains protein 3 monomer, 87 kDa Rattus norvegicus protein
Buffer: 100mM NaH2PO4, 100mM NaCl, 0.5mM DTT,, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Jun 3
RgGuinier 4.1 nm
Dmax 13.8 nm
VolumePorod 175 nm3