Ferritin-based fusion protein shows octameric deadlock state of self-assembly

Sudarev V, Gette M, Bazhenov S, Tilinova O, Zinovev E, Manukhov I, Kuklin A, Ryzhykau Y, Vlasov A
Biochemical and Biophysical Research Communications 690:149276 (2024 Jan)

doi: 10.1016/j.bbrc.2023.149276

Submitted to SASBDB: 2023 Nov 13 Published in SASBDB: 2023 Nov 24

SASDTU4 – 24×FerSUMO - Chimeric protein complex of ferritin from Helicobacter pylori fused to an N-terminal His6-SUMO protein tag Sample: Bacterial non-heme ferritin (N19Q, I59V, N-terminal His-SUMO fusion) 24-mer, 755 kDa Helicobacter pylori (strain … protein Buffer: 25 mM Tris, 150 mM NaCl, pH: 7.4 Experiment: SAXS data collected at Rigaku MicroMax 007-HF, Moscow Institute of Physics and Technology (MIPT) on 2022 Jul 30 RgGuinier 7.6 nm Dmax 25.0 nm VolumePorod 2010 nm3

SASDTV4 – 8×FerSUMO - Chimeric protein complex of ferritin from Helicobacter pylori fused to an N-terminal His6-SUMO protein tag Sample: Bacterial non-heme ferritin (N19Q, I59V, N-terminal His-SUMO fusion) octamer, 252 kDa Helicobacter pylori (strain … protein Buffer: 25 mM Tris, 150 mM NaCl, pH: 7.4 Experiment: SAXS data collected at Rigaku MicroMax 007-HF, Moscow Institute of Physics and Technology (MIPT) on 2022 Jul 17 RgGuinier 5.4 nm Dmax 16.0 nm VolumePorod 618 nm3

SASDTW4 – Ferritin from Helicobacter pylori with an N-terminal His6-tag Sample: Bacterial non-heme ferritin (N19Q, I59V) 24-mer, 480 kDa Helicobacter pylori (strain … protein Buffer: 20 mM Tris, pH: 8 Experiment: SAXS data collected at Rigaku MicroMax 007-HF, Moscow Institute of Physics and Technology (MIPT) on 2022 Dec 14 RgGuinier 5.6 nm Dmax 14.5 nm VolumePorod 674 nm3