Protein quaternary structures in solution are a mixture of multiple forms

Marciano S, Dey D, Listov D, Fleishman S, Sonn-Segev A, Mertens H, Busch F, Kim Y, Harvey S, Wysocki V, Schreiber G
Chemical Science 13(39):11680-11695 (2022)

doi: 10.1039/D2SC02794A
Submitted to SASBDB: 2021 Apr 30
Published in SASBDB:

SASDLR4 – 3-oxoacyl-[acyl-carrier-protein] reductase, FabG

3-oxoacyl-[acyl-carrier-protein] reductase FabG experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: 3-oxoacyl-[acyl-carrier-protein] reductase FabG, 26 kDa Escherichia coli (strain … protein
Buffer: 50 mM HEPES, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Oct 1
RgGuinier 3.6 nm
Dmax 10.6 nm
VolumePorod 212 nm3

SASDLQ4 – Deoxyribose-phosphate aldolase, DeoC

Deoxyribose-phosphate aldolase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Deoxyribose-phosphate aldolase, 28 kDa Escherichia coli (strain … protein
Buffer: 50 mM HEPES, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Oct 1
RgGuinier 2.6 nm
Dmax 8.0 nm
VolumePorod 61 nm3

SASDLP4 – Superoxide dismutase, SodA

Superoxide dismutase [Mn] experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Superoxide dismutase [Mn], 23 kDa Escherichia coli (strain … protein
Buffer: 50 mM HEPES, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Oct 1
RgGuinier 2.3 nm
Dmax 7.3 nm
VolumePorod 54 nm3

SASDMT3 – NAD Kinase

NAD kinase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: NAD kinase, 33 kDa Escherichia coli (strain … protein
Buffer: 50 mM HEPES, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Oct 2
RgGuinier 4.0 nm
Dmax 12.5 nm
VolumePorod 260 nm3