Assembly of higher-order SMN oligomers is essential for metazoan viability and requires an exposed structural motif present in the YG zipper dimer.

Gupta K, Wen Y, Ninan NS, Raimer AC, Sharp R, Spring AM, Sarachan KL, Johnson MC, Van Duyne GD, Matera AG
Nucleic Acids Res 49(13):7644-7664 (2021 Jul 21)
PMID: 34181727
doi: 10.1093/nar/gkab508 		Submitted to SASBDB: 2021 May 14
Published in SASBDB:

SASDL35 – Schizosaccharomyces pombe SMN(GCN4IL) Chimera•Gemin2 complex (12 mg/mL)

Survival motor neuron-like protein 1Survival of motor neuron protein-interacting protein yip11Gcn4p experimental SAS data
Survival motor neuron-like protein 1 Survival of motor neuron protein-interacting protein yip11 Gcn4p Kratky plot
Sample: Survival motor neuron-like protein 1 dimer, 26 kDa Schizosaccharomyces pombe (strain … protein
Survival of motor neuron protein-interacting protein yip11 dimer, 54 kDa Schizosaccharomyces pombe (strain … protein
Gcn4p dimer, 8 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 20 mM Na/KPO4 pH 7.0, 150 mM NaCl, 1 mM DTT, pH: 7
Experiment: SAXS data collected at X21, National Synchrotron Light Source (NSLS) on 2011 Oct 21
RgGuinier 6.6 nm
Dmax 23.3 nm
VolumePorod 320 nm3

SASDL45 – Schizosaccharomyces pombe SMN(GCN4IL) Chimera•Gemin2 complex (6 mg/mL)

Survival motor neuron-like protein 1Survival of motor neuron protein-interacting protein yip11Gcn4p experimental SAS data
Survival motor neuron-like protein 1 Survival of motor neuron protein-interacting protein yip11 Gcn4p Kratky plot
Sample: Survival motor neuron-like protein 1 dimer, 26 kDa Schizosaccharomyces pombe (strain … protein
Survival of motor neuron protein-interacting protein yip11 dimer, 54 kDa Schizosaccharomyces pombe (strain … protein
Gcn4p dimer, 8 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 20 mM Na/KPO4 pH 7.0, 150 mM NaCl, 1 mM DTT, pH: 7
Experiment: SAXS data collected at X21, National Synchrotron Light Source (NSLS) on 2011 Oct 21
RgGuinier 6.6 nm
Dmax 22.9 nm
VolumePorod 325 nm3

SASDL55 – Schizosaccharomyces pombe SMN(GCN4IL) Chimera•Gemin2 complex (4 mg/mL)

Survival motor neuron-like protein 1Survival of motor neuron protein-interacting protein yip11Gcn4p experimental SAS data
Survival motor neuron-like protein 1 Survival of motor neuron protein-interacting protein yip11 Gcn4p Kratky plot
Sample: Survival motor neuron-like protein 1 dimer, 26 kDa Schizosaccharomyces pombe (strain … protein
Survival of motor neuron protein-interacting protein yip11 dimer, 54 kDa Schizosaccharomyces pombe (strain … protein
Gcn4p dimer, 8 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 20 mM Na/KPO4 pH 7.0, 150 mM NaCl, 1 mM DTT, pH: 7
Experiment: SAXS data collected at X21, National Synchrotron Light Source (NSLS) on 2011 Oct 21
RgGuinier 6.4 nm
Dmax 21.9 nm
VolumePorod 315 nm3

SASDL65 – Schizosaccharomyces pombe SMN(GCN4IL) Chimera•Gemin2 complex (3 mg/mL)

Survival motor neuron-like protein 1Survival of motor neuron protein-interacting protein yip11Gcn4p experimental SAS data
Survival motor neuron-like protein 1 Survival of motor neuron protein-interacting protein yip11 Gcn4p Kratky plot
Sample: Survival motor neuron-like protein 1 dimer, 26 kDa Schizosaccharomyces pombe (strain … protein
Survival of motor neuron protein-interacting protein yip11 dimer, 54 kDa Schizosaccharomyces pombe (strain … protein
Gcn4p dimer, 8 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 20 mM Na/KPO4 pH 7.0, 150 mM NaCl, 1 mM DTT, pH: 7
Experiment: SAXS data collected at X21, National Synchrotron Light Source (NSLS) on 2011 Oct 21
RgGuinier 6.3 nm
Dmax 19.9 nm
VolumePorod 315 nm3

SASDL75 – Maltose Binding Protein (MBP) fused to Drosophila Melanogaster SMN186–220: Tetramer analyzed by SVD-EFA SEC-SAXS

Survival motor neuron proteinMaltose/maltodextrin-binding periplasmic protein experimental SAS data
Survival motor neuron protein Maltose/maltodextrin-binding periplasmic protein Kratky plot
Sample: Survival motor neuron protein tetramer, 15 kDa Drosophila melanogaster protein
Maltose/maltodextrin-binding periplasmic protein tetramer, 174 kDa Escherichia coli (strain … protein
Buffer: 20 mM Na/KPO4 pH 7.0, 300 mM NaCl, 1 mM DTT, pH: 7
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Dec 19
RgGuinier 3.9 nm
Dmax 11.3 nm
VolumePorod 340 nm3

SASDL85 – Maltose Binding Protein (MBP) fused to Drosophila Melanogaster SMN186–220: Octamer analyzed by SVD-EFA SEC-SAXS

Maltose/maltodextrin-binding periplasmic proteinSurvival motor neuron protein experimental SAS data
Maltose/maltodextrin-binding periplasmic protein Survival motor neuron protein Kratky plot
Sample: Maltose/maltodextrin-binding periplasmic protein octamer, 347 kDa Escherichia coli (strain … protein
Survival motor neuron protein octamer, 31 kDa Drosophila melanogaster protein
Buffer: 20 mM Na/KPO4 pH 7.0, 300 mM NaCl, 1 mM DTT, pH: 7
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Dec 19
RgGuinier 5.6 nm
Dmax 17.9 nm
VolumePorod 800 nm3

SASDL95 – Drosophila Melanogaster Gemin2-SMN: Octamer analyzed by SVD-EFA SEC-SAXS

Protein Gemin2Survival motor neuron protein experimental SAS data
Protein Gemin2 Survival motor neuron protein Kratky plot
Sample: Protein Gemin2 octamer, 230 kDa Drosophila melanogaster protein
Survival motor neuron protein octamer, 197 kDa Drosophila melanogaster protein
Buffer: 20 mM Na/KPO4 pH 7.0, 300 mM NaCl, 1 mM DTT, pH: 7
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Dec 19
RgGuinier 7.8 nm
Dmax 28.7 nm
VolumePorod 2660 nm3