Wing phosphorylation is a major functional determinant of the Lrs14-type biofilm and motility regulator AbfR1 in Sulfolobus acidocaldarius.

Li L, Banerjee A, Bischof LF, Maklad HR, Hoffmann L, Henche AL, Veliz F, Bildl W, Schulte U, Orell A, Essen LO, Peeters E, Albers SV
Mol Microbiol 105(5):777-793 (2017 Sep)

PMID: 28628237 doi: 10.1111/mmi.13735

Submitted to SASBDB: 2015 Oct 1 Published in SASBDB: 2017 May 23

SASDBF2 – Solution structure of 40bp long dsDNA-Sa Oligonucleotide Sample: 40bp long dsDNA-Sa Oligonucleotide monomer, 25 kDa DNA Buffer: 0.5 x Tris/Borate/EDTA (TBE), pH: Experiment: SAXS data collected at BM29, ESRF on 2015 Aug 28 RgGuinier 3.6 nm Dmax 12.0 nm

SASDBG2 – Wild-type archaeal biofilm regulator 1 (ABfR1: Transcriptional regulator ArsR family). Sample: Wild-type archaeal biofilm regulator 1 (ABfR1: Transcriptional regulator ArsR family). dimer, 26 kDa Sulfolobus acidocaldarius protein Buffer: 20 mM HEPES 200 mM NaCl, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2015 Aug 27 RgGuinier 2.6 nm Dmax 9.8 nm VolumePorod 47 nm3

SASDBH2 – Mutant archaeal biofilm regulator 1 (ABfR1) phosphomimic Y84E S87D Sample: Archaeal biofilm regulator 1 (AbfR1) mutant Y84E S87D phosphomimic mutant dimer, 26 kDa Sulfolobus acidocaldarius protein Buffer: 20 mM HEPES 200 mM NaCl, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2015 Aug 27 RgGuinier 2.6 nm Dmax 9.5 nm VolumePorod 51 nm3

SASDBJ2 – Wild-type archaeal biofilm regulator 1 (ABfR1) bound to 40bp dsDNA-Sa oligonucleotide. Sample: 40bp long dsDNA-Sa Oligonucleotide monomer, 25 kDa DNA Wild-type archaeal biofilm regulator 1 (ABfR1: Transcriptional regulator ArsR family). dimer, 26 kDa Sulfolobus acidocaldarius protein Buffer: 0.5 x Tris/Borate/EDTA (TBE), pH: Experiment: SAXS data collected at BM29, ESRF on 2015 Aug 27 RgGuinier 3.0 nm Dmax 11.8 nm