Structural insights into the contactin 1 – neurofascin 155 adhesion complex
Chataigner L,
Gogou C,
den Boer M,
Frias C,
Thies-Weesie D,
Granneman J,
Heck A,
Meijer D,
Janssen B
Nature Communications
13(1)
(2022 Nov 03)
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Sample: |
Neurofascin dimer, 139 kDa Mus musculus protein
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Buffer: |
25 mM HEPES, 150 mM NaCl, pH: 7.5 |
Experiment: |
SAXS
data collected at B21, Diamond Light Source on 2019 Dec 16
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|
RgGuinier |
4.7 |
nm |
Dmax |
18.0 |
nm |
VolumePorod |
355 |
nm3 |
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|
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Sample: |
Neurofascin dimer, 139 kDa Mus musculus protein
|
Buffer: |
25 mM HEPES, 150 mM NaCl, pH: 7.5 |
Experiment: |
SAXS
data collected at B21, Diamond Light Source on 2019 Dec 16
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|
RgGuinier |
5.0 |
nm |
Dmax |
21.0 |
nm |
VolumePorod |
450 |
nm3 |
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|
|
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Sample: |
Neurofascin dimer, 139 kDa Mus musculus protein
|
Buffer: |
25 mM HEPES, 150 mM NaCl, pH: 7.5 |
Experiment: |
SAXS
data collected at B21, Diamond Light Source on 2019 Dec 16
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|
RgGuinier |
4.5 |
nm |
Dmax |
17.0 |
nm |
VolumePorod |
290 |
nm3 |
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|
|
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|
Sample: |
Neurofascin dimer, 139 kDa Mus musculus protein
|
Buffer: |
25 mM HEPES, 150 mM NaCl, pH: 7.5 |
Experiment: |
SAXS
data collected at B21, Diamond Light Source on 2019 Dec 16
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|
RgGuinier |
4.3 |
nm |
Dmax |
17.0 |
nm |
VolumePorod |
240 |
nm3 |
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|
|
|
|
Sample: |
Neurofascin dimer, 139 kDa Mus musculus protein
|
Buffer: |
25 mM HEPES, 150 mM NaCl, pH: 7.5 |
Experiment: |
SAXS
data collected at B21, Diamond Light Source on 2019 Dec 16
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|
RgGuinier |
4.2 |
nm |
Dmax |
17.0 |
nm |
VolumePorod |
207 |
nm3 |
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|
|
|
|
Sample: |
Neurofascin dimer, 139 kDa Mus musculus protein
|
Buffer: |
25 mM HEPES, 150 mM NaCl, pH: 7.5 |
Experiment: |
SAXS
data collected at B21, Diamond Light Source on 2019 Dec 16
|
|
RgGuinier |
4.1 |
nm |
Dmax |
17.0 |
nm |
VolumePorod |
187 |
nm3 |
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|
|
|
Sample: |
Neurofascin dimer, 139 kDa Mus musculus protein
|
Buffer: |
25 mM HEPES, 150 mM NaCl, pH: 7.5 |
Experiment: |
SAXS
data collected at B21, Diamond Light Source on 2019 Dec 16
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|
RgGuinier |
3.9 |
nm |
Dmax |
12.6 |
nm |
VolumePorod |
172 |
nm3 |
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Sample: |
Neurofascin T216A monomer, 70 kDa Mus musculus protein
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Buffer: |
25 mM HEPES, 150 mM NaCl, pH: 7.5 |
Experiment: |
SAXS
data collected at B21, Diamond Light Source on 2020 Jun 25
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RgGuinier |
4.3 |
nm |
Dmax |
18.0 |
nm |
VolumePorod |
212 |
nm3 |
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Sample: |
Neurofascin T216A monomer, 70 kDa Mus musculus protein
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Buffer: |
25 mM HEPES, 150 mM NaCl, pH: 7.5 |
Experiment: |
SAXS
data collected at B21, Diamond Light Source on 2020 Jun 25
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RgGuinier |
3.9 |
nm |
Dmax |
16.0 |
nm |
VolumePorod |
158 |
nm3 |
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Sample: |
Neurofascin T216A monomer, 70 kDa Mus musculus protein
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Buffer: |
25 mM HEPES, 150 mM NaCl, pH: 7.5 |
Experiment: |
SAXS
data collected at B21, Diamond Light Source on 2020 Jun 25
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RgGuinier |
3.8 |
nm |
Dmax |
14.0 |
nm |
VolumePorod |
150 |
nm3 |
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Sample: |
Neurofascin T216A monomer, 70 kDa Mus musculus protein
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Buffer: |
25 mM HEPES, 150 mM NaCl, pH: 7.5 |
Experiment: |
SAXS
data collected at B21, Diamond Light Source on 2020 Jun 25
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|
RgGuinier |
3.6 |
nm |
Dmax |
10.6 |
nm |
VolumePorod |
139 |
nm3 |
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Sample: |
Contactin-1 I433V dimer, 134 kDa Mus musculus protein
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Buffer: |
25 mM HEPES, 150 mM NaCl, pH: 7.5 |
Experiment: |
SAXS
data collected at B21, Diamond Light Source on 2019 Dec 16
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|
RgGuinier |
5.0 |
nm |
Dmax |
23.0 |
nm |
VolumePorod |
180 |
nm3 |
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Sample: |
Contactin-1 I433V dimer, 134 kDa Mus musculus protein
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Buffer: |
25 mM HEPES, 150 mM NaCl, pH: 7.5 |
Experiment: |
SAXS
data collected at B21, Diamond Light Source on 2019 Dec 16
|
|
RgGuinier |
4.9 |
nm |
Dmax |
23.0 |
nm |
VolumePorod |
166 |
nm3 |
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Sample: |
Contactin-1 I433V dimer, 134 kDa Mus musculus protein
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Buffer: |
25 mM HEPES, 150 mM NaCl, pH: 7.5 |
Experiment: |
SAXS
data collected at B21, Diamond Light Source on 2019 Dec 16
|
|
RgGuinier |
4.9 |
nm |
Dmax |
23.0 |
nm |
VolumePorod |
155 |
nm3 |
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Sample: |
Contactin-1 I433V dimer, 134 kDa Mus musculus protein
|
Buffer: |
25 mM HEPES, 150 mM NaCl, pH: 7.5 |
Experiment: |
SAXS
data collected at B21, Diamond Light Source on 2019 Dec 16
|
|
RgGuinier |
4.9 |
nm |
Dmax |
15.8 |
nm |
VolumePorod |
118 |
nm3 |
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|
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|
Sample: |
Contactin-1 I433V dimer, 134 kDa Mus musculus protein
|
Buffer: |
25 mM HEPES, 150 mM NaCl, pH: 7.5 |
Experiment: |
SAXS
data collected at B21, Diamond Light Source on 2019 Dec 16
|
|
RgGuinier |
5.2 |
nm |
Dmax |
23.0 |
nm |
VolumePorod |
154 |
nm3 |
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|
Sample: |
Contactin-1 I433V dimer, 134 kDa Mus musculus protein
|
Buffer: |
25 mM HEPES, 150 mM NaCl, pH: 7.5 |
Experiment: |
SAXS
data collected at B21, Diamond Light Source on 2019 Dec 16
|
|
RgGuinier |
5.0 |
nm |
Dmax |
23.0 |
nm |
VolumePorod |
150 |
nm3 |
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|
|
|
|
Sample: |
Contactin-1 I433V dimer, 134 kDa Mus musculus protein
|
Buffer: |
25 mM HEPES, 150 mM NaCl, pH: 7.5 |
Experiment: |
SAXS
data collected at B21, Diamond Light Source on 2019 Dec 16
|
|
RgGuinier |
5.0 |
nm |
Dmax |
23.0 |
nm |
VolumePorod |
146 |
nm3 |
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|
|
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|
Sample: |
Contactin-1 I433V dimer, 134 kDa Mus musculus protein
|
Buffer: |
25 mM HEPES, 150 mM NaCl, pH: 7.5 |
Experiment: |
SAXS
data collected at B21, Diamond Light Source on 2019 Dec 16
|
|
RgGuinier |
4.9 |
nm |
Dmax |
14.5 |
nm |
VolumePorod |
100 |
nm3 |
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Sample: |
Contactin-1 I433V dimer, 220 kDa Mus musculus protein
|
Buffer: |
25 mM HEPES, 150 mM NaCl, pH: 7.5 |
Experiment: |
SAXS
data collected at B21, Diamond Light Source on 2019 Dec 16
|
|
RgGuinier |
8.1 |
nm |
Dmax |
42.0 |
nm |
VolumePorod |
520 |
nm3 |
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Sample: |
Contactin-1 I433V dimer, 220 kDa Mus musculus protein
|
Buffer: |
25 mM HEPES, 150 mM NaCl, pH: 7.5 |
Experiment: |
SAXS
data collected at B21, Diamond Light Source on 2019 Dec 16
|
|
RgGuinier |
8.0 |
nm |
Dmax |
43.0 |
nm |
VolumePorod |
530 |
nm3 |
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|
|
|
|
Sample: |
Contactin-1 I433V dimer, 220 kDa Mus musculus protein
|
Buffer: |
25 mM HEPES, 150 mM NaCl, pH: 7.5 |
Experiment: |
SAXS
data collected at B21, Diamond Light Source on 2019 Dec 16
|
|
RgGuinier |
8.0 |
nm |
Dmax |
43.0 |
nm |
VolumePorod |
530 |
nm3 |
|
|
|
|
|
Sample: |
Contactin-1 I433V dimer, 220 kDa Mus musculus protein
|
Buffer: |
25 mM HEPES, 150 mM NaCl, pH: 7.5 |
Experiment: |
SAXS
data collected at B21, Diamond Light Source on 2019 Dec 16
|
|
RgGuinier |
8.0 |
nm |
Dmax |
25.7 |
nm |
VolumePorod |
350 |
nm3 |
|
|
|
|
|
Sample: |
Contactin-1 I433V dimer, 220 kDa Mus musculus protein
|
Buffer: |
25 mM HEPES, 150 mM NaCl, pH: 7.5 |
Experiment: |
SAXS
data collected at B21, Diamond Light Source on 2019 Dec 16
|
|
RgGuinier |
7.0 |
nm |
Dmax |
34.0 |
nm |
VolumePorod |
285 |
nm3 |
|
|
|
|
|
Sample: |
Contactin-1 I433V dimer, 220 kDa Mus musculus protein
|
Buffer: |
25 mM HEPES, 150 mM NaCl, pH: 7.5 |
Experiment: |
SAXS
data collected at B21, Diamond Light Source on 2019 Dec 16
|
|
RgGuinier |
6.8 |
nm |
Dmax |
27.0 |
nm |
VolumePorod |
275 |
nm3 |
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|
Sample: |
Contactin-1 I433V dimer, 220 kDa Mus musculus protein
|
Buffer: |
25 mM HEPES, 150 mM NaCl, pH: 7.5 |
Experiment: |
SAXS
data collected at B21, Diamond Light Source on 2019 Dec 16
|
|
RgGuinier |
6.8 |
nm |
Dmax |
27.0 |
nm |
VolumePorod |
270 |
nm3 |
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|
|
|
|
Sample: |
Contactin-1 I433V dimer, 220 kDa Mus musculus protein
|
Buffer: |
25 mM HEPES, 150 mM NaCl, pH: 7.5 |
Experiment: |
SAXS
data collected at B21, Diamond Light Source on 2019 Dec 16
|
|
RgGuinier |
6.8 |
nm |
Dmax |
19.5 |
nm |
VolumePorod |
254 |
nm3 |
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