Structural insights into the contactin 1 – neurofascin 155 adhesion complex

Chataigner L, Gogou C, den Boer M, Frias C, Thies-Weesie D, Granneman J, Heck A, Meijer D, Janssen B
Nature Communications 13(1) (2022 Nov 03)

doi: 10.1038/s41467-022-34302-9
Submitted to SASBDB: 2021 May 24
Published in SASBDB:

SASDL66 – High mannose neurofascin 155 immunoglobulin domains 1-6, 37.2 μM

Neurofascin experimental SAS data
Neurofascin Kratky plot
Sample: Neurofascin dimer, 139 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
RgGuinier 4.7 nm
Dmax 18.0 nm
VolumePorod 355 nm3

SASDL76 – High mannose neurofascin 155 immunoglobulin domains 1-6, 73.5 μM

Neurofascin experimental SAS data
Neurofascin Kratky plot
Sample: Neurofascin dimer, 139 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
RgGuinier 5.0 nm
Dmax 21.0 nm
VolumePorod 450 nm3

SASDL86 – High mannose neurofascin 155 immunoglobulin domains 1-6, 19.7 μM

Neurofascin experimental SAS data
Neurofascin Kratky plot
Sample: Neurofascin dimer, 139 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
RgGuinier 4.5 nm
Dmax 17.0 nm
VolumePorod 290 nm3

SASDL96 – High mannose neurofascin 155 immunoglobulin domains 1-6, 9.5 μM

Neurofascin experimental SAS data
Neurofascin Kratky plot
Sample: Neurofascin dimer, 139 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
RgGuinier 4.3 nm
Dmax 17.0 nm
VolumePorod 240 nm3

SASDLA6 – High mannose neurofascin 155 immunoglobulin domains 1-6, 5.1 μM

Neurofascin experimental SAS data
Neurofascin Kratky plot
Sample: Neurofascin dimer, 139 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
RgGuinier 4.2 nm
Dmax 17.0 nm
VolumePorod 207 nm3

SASDLB6 – High mannose neurofascin 155 immunoglobulin domains 1-6, 2.7 μM

Neurofascin experimental SAS data
Neurofascin Kratky plot
Sample: Neurofascin dimer, 139 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
RgGuinier 4.1 nm
Dmax 17.0 nm
VolumePorod 187 nm3

SASDLC6 – High mannose neurofascin 155 immunoglobulin domains 1-6, 1.3 μM

Neurofascin experimental SAS data
Neurofascin Kratky plot
Sample: Neurofascin dimer, 139 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
RgGuinier 3.9 nm
Dmax 12.6 nm
VolumePorod 172 nm3

SASDLD6 – High mannose neurofascin 155 immunoglobulin domains 1-6 Thr216Ala, 109.3 μM

Neurofascin T216A experimental SAS data
Neurofascin T216A Kratky plot
Sample: Neurofascin T216A monomer, 70 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Jun 25
RgGuinier 4.3 nm
Dmax 18.0 nm
VolumePorod 212 nm3

SASDLE6 – High mannose neurofascin 155 immunoglobulin domains 1-6 Thr216Ala, 21.9 μM

Neurofascin T216A experimental SAS data
Neurofascin T216A Kratky plot
Sample: Neurofascin T216A monomer, 70 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Jun 25
RgGuinier 3.9 nm
Dmax 16.0 nm
VolumePorod 158 nm3

SASDLF6 – High mannose neurofascin 155 immunoglobulin domains 1-6 Thr216Ala, 5.5 μM

Neurofascin T216A experimental SAS data
Neurofascin T216A Kratky plot
Sample: Neurofascin T216A monomer, 70 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Jun 25
RgGuinier 3.8 nm
Dmax 14.0 nm
VolumePorod 150 nm3

SASDLG6 – High mannose neurofascin 155 immunoglobulin domains 1-6 Thr216Ala, 1.1 μM

Neurofascin T216A experimental SAS data
Neurofascin T216A Kratky plot
Sample: Neurofascin T216A monomer, 70 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Jun 25
RgGuinier 3.6 nm
Dmax 10.6 nm
VolumePorod 139 nm3

SASDLH6 – Complex glycan contactin 1 immunoglobulin domains 1-6, 24.6 μM

Contactin-1 I433V experimental SAS data
Contactin-1 I433V Kratky plot
Sample: Contactin-1 I433V dimer, 134 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
RgGuinier 5.0 nm
Dmax 23.0 nm
VolumePorod 180 nm3

SASDLJ6 – Complex glycan contactin 1 immunoglobulin domains 1-6, 12.3 μM

Contactin-1 I433V experimental SAS data
Contactin-1 I433V Kratky plot
Sample: Contactin-1 I433V dimer, 134 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
RgGuinier 4.9 nm
Dmax 23.0 nm
VolumePorod 166 nm3

SASDLK6 – Complex glycan contactin 1 immunoglobulin domains 1-6, 6.2 μM

Contactin-1 I433V experimental SAS data
Contactin-1 I433V Kratky plot
Sample: Contactin-1 I433V dimer, 134 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
RgGuinier 4.9 nm
Dmax 23.0 nm
VolumePorod 155 nm3

SASDLL6 – Complex glycan contactin 1 immunoglobulin domains 1-6, 3.1 μM

Contactin-1 I433V experimental SAS data
Contactin-1 I433V Kratky plot
Sample: Contactin-1 I433V dimer, 134 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
RgGuinier 4.9 nm
Dmax 15.8 nm
VolumePorod 118 nm3

SASDLM6 – High mannose glycan contactin 1 immunoglobulin domains 1-6, 21.8 μM

Contactin-1 I433V experimental SAS data
Contactin-1 I433V Kratky plot
Sample: Contactin-1 I433V dimer, 134 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
RgGuinier 5.2 nm
Dmax 23.0 nm
VolumePorod 154 nm3

SASDLN6 – High mannose glycan contactin 1 immunoglobulin domains 1-6, 10.9 μM

Contactin-1 I433V experimental SAS data
Contactin-1 I433V Kratky plot
Sample: Contactin-1 I433V dimer, 134 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
RgGuinier 5.0 nm
Dmax 23.0 nm
VolumePorod 150 nm3

SASDLP6 – High mannose glycan contactin 1 immunoglobulin domains 1-6, 5.5 μM

Contactin-1 I433V experimental SAS data
Contactin-1 I433V Kratky plot
Sample: Contactin-1 I433V dimer, 134 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
RgGuinier 5.0 nm
Dmax 23.0 nm
VolumePorod 146 nm3

SASDLQ6 – High mannose glycan contactin 1 immunoglobulin domains 1-6, 2.7 μM

Contactin-1 I433V experimental SAS data
CUSTOM IN-HOUSE model
Sample: Contactin-1 I433V dimer, 134 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
RgGuinier 4.9 nm
Dmax 14.5 nm
VolumePorod 100 nm3

SASDLR6 – Complex glycan contactin 1 ectodomain, 20 μM

Contactin-1 I433V experimental SAS data
Contactin-1 I433V Kratky plot
Sample: Contactin-1 I433V dimer, 220 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
RgGuinier 8.1 nm
Dmax 42.0 nm
VolumePorod 520 nm3

SASDLS6 – Complex glycan contactin 1 ectodomain, 10 μM

Contactin-1 I433V experimental SAS data
Contactin-1 I433V Kratky plot
Sample: Contactin-1 I433V dimer, 220 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
RgGuinier 8.0 nm
Dmax 43.0 nm
VolumePorod 530 nm3

SASDLT6 – Complex glycan contactin 1 ectodomain, 5 μM

Contactin-1 I433V experimental SAS data
Contactin-1 I433V Kratky plot
Sample: Contactin-1 I433V dimer, 220 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
RgGuinier 8.0 nm
Dmax 43.0 nm
VolumePorod 530 nm3

SASDLU6 – Complex glycan contactin 1 ectodomain, 2.5 μM

Contactin-1 I433V experimental SAS data
Contactin-1 I433V Kratky plot
Sample: Contactin-1 I433V dimer, 220 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
RgGuinier 8.0 nm
Dmax 25.7 nm
VolumePorod 350 nm3

SASDLV6 – High mannose glycan contactin 1 ectodomain, 20.5 μM

Contactin-1 I433V experimental SAS data
Contactin-1 I433V Kratky plot
Sample: Contactin-1 I433V dimer, 220 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
RgGuinier 7.0 nm
Dmax 34.0 nm
VolumePorod 285 nm3

SASDLW6 – High mannose glycan contactin 1 ectodomain, 11.1 μM

Contactin-1 I433V experimental SAS data
Contactin-1 I433V Kratky plot
Sample: Contactin-1 I433V dimer, 220 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
RgGuinier 6.8 nm
Dmax 27.0 nm
VolumePorod 275 nm3

SASDLX6 – High mannose glycan contactin 1 ectodomain, 5.5 μM

Contactin-1 I433V experimental SAS data
Contactin-1 I433V Kratky plot
Sample: Contactin-1 I433V dimer, 220 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
RgGuinier 6.8 nm
Dmax 27.0 nm
VolumePorod 270 nm3

SASDLY6 – High mannose glycan contactin 1 ectodomain, 2.4 μM

Contactin-1 I433V experimental SAS data
CUSTOM IN-HOUSE model
Sample: Contactin-1 I433V dimer, 220 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
RgGuinier 6.8 nm
Dmax 19.5 nm
VolumePorod 254 nm3