A structural study of the cytoplasmic chaperone effect of 14-3-3 proteins on Ataxin-1.

Leysen S, Jane Burnley R, Rodriguez E, Milroy LG, Soini L, Adamski CJ, Nitschke L, Davis R, Obsil T, Brunsveld L, Crabbe T, Yahya Zoghbi H, Ottmann C, Martin Davis J
J Mol Biol :167174 (2021 Jul 21)

PMID: 34302818 doi: 10.1016/j.jmb.2021.167174

Submitted to SASBDB: 2021 Jun 3 Published in SASBDB: 2021 Jul 28

SASDL56 – 14-3-3 zeta truncated at C-terminus Sample: 14-3-3 protein zeta/delta dimer, 53 kDa Homo sapiens protein Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM DTT, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Oct 13 RgGuinier 2.8 nm Dmax 7.9 nm

SASDLT3 – Ataxin-1 AXH-C Sample: Ataxin-1 dimer, 55 kDa Homo sapiens protein Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM DTT, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Oct 13 RgGuinier 4.3 nm Dmax 14.6 nm VolumePorod 90 nm3

SASDL46 – 14-3-3zeta AXH-C complex Sample: 14-3-3 protein zeta/delta dimer, 53 kDa Homo sapiens protein Ataxin-1 AXH-C dimer, 55 kDa Homo sapiens protein Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM DTT, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Oct 13 RgGuinier 4.8 nm Dmax 19.8 nm