Structure and function of Bartonella effector protein 1: target and interdomain interactions

Markus Huber, Jens Reiners
University of Basel PhD thesis 15051 (2023 Jul 03)

URL: https://edoc.unibas.ch/94460/ 		Submitted to SASBDB: 2021 Jun 28
Published in SASBDB:

SASDLK7 – Bartonella effector protein (Bep1), 15 °C

Bartonella effector protein (Bep) substrate of VirB T4SS experimental SAS data
GASBOR model
Sample: Bartonella effector protein (Bep) substrate of VirB T4SS monomer, 64 kDa Bartonella clarridgeiae (strain … protein
Buffer: 25 mM Hepes, 300 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.5
Experiment: SAXS data collected at Xenocs Xeuss 2.0 Q-Xoom, Center for Structural Studies, Heinrich-Heine-University on 2020 May 29
RgGuinier 4.1 nm
Dmax 13.4 nm
VolumePorod 83 nm3

SASDLL7 – Bartonella effector protein (Bep1), 20 °C

Bartonella effector protein (Bep) substrate of VirB T4SS experimental SAS data
GASBOR model
Sample: Bartonella effector protein (Bep) substrate of VirB T4SS monomer, 64 kDa Bartonella clarridgeiae (strain … protein
Buffer: 25 mM Hepes, 300 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 26
RgGuinier 3.8 nm
Dmax 13.4 nm
VolumePorod 87 nm3

SASDLM7 – Bartonella effector protein (Bep1), 35 °C

Bartonella effector protein (Bep) substrate of VirB T4SS experimental SAS data
GASBOR model
Sample: Bartonella effector protein (Bep) substrate of VirB T4SS monomer, 64 kDa Bartonella clarridgeiae (strain … protein
Buffer: 25 mM Hepes, 300 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 26
RgGuinier 4.1 nm
Dmax 14.4 nm
VolumePorod 108 nm3