Radiation resistivity of clISCA1

Shigeki Arai

Submitted to SASBDB: 2021 Jul 5 Published in SASBDB: 2024 Jul 5

SASDLT7 – The Fe–S cluster assembly 1 homolog of pigeon (29.3 mg/ml), Total X-ray exposure time : 1min Sample: Iron-sulfur cluster assembly 1 homolog, mitochondrial, 15 kDa Columba livia protein Buffer: 20 mM Tris-HCl, 0.15 M NaCl, 10 mM 3-mercapto-1,2-propanediol, pH: 8 Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2020 Nov 30 RgGuinier 4.3 nm Dmax 15.8 nm

SASDLU7 – The Fe–S cluster assembly 1 homolog of pigeon (29.3 mg/ml), Total X-ray exposure time : 2min Sample: Pigeon iron-sulfur cluster assembly 1 homolog, mitochondrial, 15 kDa Columba livia protein Buffer: 20 mM Tris-HCl, 0.15 M NaCl, 10 mM 3-mercapto-1,2-propanediol, pH: 8 Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2020 Nov 30 RgGuinier 4.4 nm

SASDLV7 – The Fe–S cluster assembly 1 homolog of pigeon (29.3 mg/ml), Total X-ray exposure time : 3min Sample: Iron-sulfur cluster assembly 1 homolog, mitochondrial, 15 kDa Columba livia protein Buffer: 20 mM Tris-HCl, 0.15 M NaCl, 10 mM 3-mercapto-1,2-propanediol, pH: 8 Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2020 Nov 30 RgGuinier 4.6 nm

SASDLW7 – The Fe–S cluster assembly 1 homolog of pigeon (29.3 mg/ml), Total X-ray exposure time : 4min Sample: Iron-sulfur cluster assembly 1 homolog, mitochondrial, 15 kDa Columba livia protein Buffer: 20 mM Tris-HCl, 0.15 M NaCl, 10 mM 3-mercapto-1,2-propanediol, pH: 8 Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2020 Nov 30 RgGuinier 4.5 nm

SASDLX7 – The Fe–S cluster assembly 1 homolog of pigeon (29.3 mg/ml), Total X-ray exposure time : 5min Sample: Iron-sulfur cluster assembly 1 homolog, mitochondrial, 15 kDa Columba livia protein Buffer: 20 mM Tris-HCl, 0.15 M NaCl, 10 mM 3-mercapto-1,2-propanediol, pH: 8 Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2020 Nov 30 RgGuinier 4.4 nm

SASDLY7 – The Fe–S cluster assembly 1 homolog of pigeon (6.9 mg/ml), Total X-ray exposure time : 1min Sample: Iron-sulfur cluster assembly 1 homolog, mitochondrial, 15 kDa Columba livia protein Buffer: 20 mM Tris-HCl, 0.15 M NaCl, 10 mM 3-mercapto-1,2-propanediol, pH: 8 Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Jun 8 RgGuinier 2.7 nm

SASDLZ7 – The Fe–S cluster assembly 1 homolog of pigeon (6.9 mg/ml), Total X-ray exposure time : 2min Sample: Iron-sulfur cluster assembly 1 homolog, mitochondrial, 15 kDa Columba livia protein Buffer: 20 mM Tris-HCl, 0.15 M NaCl, 10 mM 3-mercapto-1,2-propanediol, pH: 8 Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Jun 8 RgGuinier 2.8 nm

SASDL28 – The Fe–S cluster assembly 1 homolog of pigeon (6.9 mg/ml), Total X-ray exposure time : 3min Sample: Iron-sulfur cluster assembly 1 homolog, mitochondrial, 15 kDa Columba livia protein Buffer: 20 mM Tris-HCl, 0.15 M NaCl, 10 mM 3-mercapto-1,2-propanediol, pH: 8 Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Jun 8 RgGuinier 2.7 nm

SASDL38 – The Fe–S cluster assembly 1 homolog of pigeon (6.9 mg/ml), Total X-ray exposure time : 4min Sample: Iron-sulfur cluster assembly 1 homolog, mitochondrial, 15 kDa Columba livia protein Buffer: 20 mM Tris-HCl, 0.15 M NaCl, 10 mM 3-mercapto-1,2-propanediol, pH: 8 Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Jun 8 RgGuinier 2.7 nm

SASDL48 – The Fe–S cluster assembly 1 homolog of pigeon (6.9 mg/ml), Total X-ray exposure time : 5min Sample: Iron-sulfur cluster assembly 1 homolog, mitochondrial, 15 kDa Columba livia protein Buffer: 20 mM Tris-HCl, 0.15 M NaCl, 10 mM 3-mercapto-1,2-propanediol, pH: 8 Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Jun 8 RgGuinier 2.7 nm