Structural and Functional Characterization of Rv0792c from Mycobacterium tuberculosis: Identifying Small Molecule Inhibitor against HutC Protein.

Chauhan NK, Anand A, Sharma A, Dhiman K, Gosain TP, Singh P, Singh P, Khan E, Chattopadhyay G, Kumar A, Sharma D, Ashish, Sharma TK, Singh R
Microbiol Spectr :e0197322 (2022 Dec 12)

PMID: 36507689 doi: 10.1128/spectrum.01973-22

Submitted to SASBDB: 2021 Jul 12 Published in SASBDB: 2022 Dec 14

SASDLS8 – Complex of Mtb GntR and Aptamer 1 [Rv0792c and Rv0792c_1] Sample: Complex of Rv0792c and Rv0792c_1 monomer, 74 kDa Buffer: 25 mM HEPES Buffer; 400 mM NaCl,, pH: 7.2 Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) on 2019 Oct 31 RgGuinier 3.6 nm Dmax 12.0 nm

SASDLT8 – Complex of Mtb GntR and Aptamer 2 [Rv0792c and Rv0792c_2] Sample: Complex of Rv0792c and Rv0792c_2 monomer, 46 kDa Buffer: 25 mM HEPES Buffer; 400 mM NaCl,, pH: 7.2 Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) on 2019 Oct 31 RgGuinier 3.1 nm Dmax 9.8 nm

SASDLU8 – Complex of Mtb GntR and Aptamer 5 [Rv0792c and Rv0792c_5] Sample: Complex of GntR protein: Aptamer5 monomer, 78 kDa Buffer: 25 mM HEPES Buffer; 400 mM NaCl,, pH: 7.2 Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) on 2019 Oct 31 RgGuinier 3.4 nm Dmax 12.0 nm

SASDLV8 – SS DNA Aptamer 5 which binds Mtb GntR Homolog [Rv0792c_5] Sample: SSDNA Aptamer from SELEX against Mtb Rv0792c monomer, 14 kDa unidentified DNA Buffer: 25 mM HEPES Buffer; 400 mM NaCl,, pH: 7.2 Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) on 2019 Oct 21 RgGuinier 1.8 nm Dmax 7.1 nm

SASDLW8 – SS DNA Aptamer 2 which binds Mtb GntR Homolog [Rv0792c_2] Sample: SSDNA Aptamer from SELEX against Mtb Rv0792c monomer, 13 kDa unidentified DNA Buffer: 25 mM HEPES Buffer; 400 mM NaCl,, pH: 7.2 Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) on 2019 Oct 20 RgGuinier 1.9 nm Dmax 7.6 nm

SASDLX8 – SS DNA Aptamer 1 which binds Mtb GntR Homolog [Rv0792c_1] Sample: SSDNA Aptamer from SELEX against Mtb Rv0792c monomer, 13 kDa unidentified DNA Buffer: 25 mM HEPES Buffer; 400 mM NaCl,, pH: 7.2 Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) on 2019 Oct 20 RgGuinier 2.1 nm Dmax 8.6 nm

SASDLY8 – Mycobacterium tuberculosis Rv0792c, a GntR homolog Sample: Recombinant Mycobacterium tuberculosis H37Rv dimer, 65 kDa Mycobacterium tuberculosis H37Rv protein Buffer: 25 mM HEPES Buffer; 400 mM NaCl,, pH: 7.2 Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) on 2019 Oct 2 RgGuinier 3.2 nm Dmax 12.0 nm

SASDLZ8 – SS DNA Aptamer 1 which binds Mtb GntR Homolog [Rv0792c_1] Sample: SSDNA Aptamer from SELEX against Mtb Rv0792c monomer, 13 kDa unidentified DNA Buffer: 25 mM HEPES Buffer; 400 mM NaCl,, pH: 7.2 Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) on 2019 Oct 20 RgGuinier 2.1 nm Dmax 8.6 nm