Experimental Evidence of Intrinsic Disorder and Amyloid Formation by the Henipavirus W Proteins

Pesce G, Gondelaud F, Ptchelkine D, Nilsson J, Bignon C, Cartalas J, Fourquet P, Longhi S
International Journal of Molecular Sciences 23(2):923 (2022 Jan 15)

doi: 10.3390/ijms23020923 		Submitted to SASBDB: 2021 Jul 15
Published in SASBDB:

SASDLK9 – Hendra virus W protein in 1 M urea, 5 mM DTT

Protein W experimental SAS data
Hendra virus W protein in 1 M urea, 5 mM DTT Rg histogram
Sample: Protein W monomer, 53 kDa Hendra virus (isolate … protein
Buffer: 20 mM HEPES, 150 mM NaCl, 1 M urea, 5 mM DTT, pH: 7
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Jun 12
RgGuinier 7.2 nm
Dmax 24.0 nm
VolumePorod 338 nm3

SASDLL9 – Nipah henipavirus W protein in 1 M urea, 5 mM DTT

Protein W experimental SAS data
Nipah henipavirus W protein in 1 M urea, 5 mM DTT Rg histogram
Sample: Protein W monomer, 53 kDa Nipah henipavirus protein
Buffer: 20 mM HEPES, 150 mM NaCl, 1 M urea, 5 mM DTT, pH: 7
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Jun 12
RgGuinier 7.1 nm
Dmax 24.5 nm
VolumePorod 327 nm3