Plasmodium falciparum Kelch13 and its artemisinin-resistant mutants assemble as hexamers in solution: a SAXS data driven modeling study.

Goel N, Dhiman K, Kalidas N, Mukhopadhyay A, Ashish F, Bhattacharjee S
FEBS J (2022 Jan 28)

PMID: 35092154 doi: 10.1111/febs.16378

Submitted to SASBDB: 2022 Feb 5 Published in SASBDB: 2022 Feb 8

SASDN85 – Truncated Kelch13-Wild Type Sample: Kelch protein K13 hexamer, 396 kDa Plasmodium falciparum (isolate … protein Buffer: phosphate buffered saline, pH: 7.4 Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) on 2020 Jul 25 RgGuinier 6.4 nm Dmax 17.0 nm VolumePorod 1630 nm3

SASDN95 – Truncated Kelch13-R539T mutant Sample: Kelch protein K13 (Truncated Kelch13-R539T, artemisinin-resistant mutation) hexamer, 396 kDa Plasmodium falciparum (isolate … protein Buffer: phosphate buffered saline, pH: 7.4 Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) on 2020 Jul 25 RgGuinier 6.6 nm Dmax 20.5 nm VolumePorod 1600 nm3

SASDNA5 – Truncated Kelch13-C580Y mutant Sample: Kelch protein K13 (Truncated Kelch13-C580Y ) hexamer, 397 kDa Plasmodium falciparum (isolate … protein Buffer: Phosphate Buffer Saline, pH: 7.4 Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) on 2020 Jul 28 RgGuinier 6.4 nm Dmax 18.4 nm VolumePorod 1800 nm3

SASDNB5 – Truncated Kelch13-A578S mutant Sample: Kelch protein K13 (Truncated Kelch13-A578S) hexamer, 396 kDa Plasmodium falciparum (isolate … protein Buffer: Phosphate Buffer Saline, pH: 7.4 Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) on 2020 Jul 29 RgGuinier 6.1 nm Dmax 16.8 nm VolumePorod 1700 nm3