Unravelling the reaction mechanism of glutamate amidation in Staphylococcus aureus peptidoglycan

Francisco Miguel Piçarra Leisico, Mertens HD
PhD thesis, NOVA University Lisbon - (2022 Jul 20)

URL: https://hdl.handle.net/10362/143893

Submitted to SASBDB: 2021 Jul 30 Published in SASBDB: 2023 Mar 20

SASDLZ9 – Streptococcus pneumoniae lipid II isoglutaminyl synthase MurT:GatD complex (no Zn2+) Sample: Lipid II isoglutaminyl synthase (glutamine-hydrolyzing) subunit MurT monomer, 52 kDa Streptococcus pneumoniae (strain … protein Lipid II isoglutaminyl synthase (glutamine-hydrolyzing) subunit GatD monomer, 29 kDa Streptococcus pneumoniae (strain … protein Buffer: 50 mM Hepes, 10 mM MgCl2, 500 mM NaCl, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jun 23 RgGuinier 3.0 nm Dmax 10.0 nm VolumePorod 126 nm3

SASDM22 – Staphylococcus aureus lipid II isoglutaminyl synthase MurT:GatD complex Sample: Lipid II isoglutaminyl synthase (glutamine-hydrolyzing) subunit MurT monomer, 49 kDa Staphylococcus aureus (strain … protein Lipid II isoglutaminyl synthase (glutamine-hydrolyzing) subunit GatD monomer, 28 kDa Staphylococcus aureus (strain … protein Buffer: 100 mM Tris-HCl, 500 mM NaCl, 10 mM MgCl2, pH: 8.5 Experiment: SAXS data collected at BM29, ESRF on 2018 Nov 29 RgGuinier 3.1 nm Dmax 10.5 nm VolumePorod 121 nm3