Solution Behavior of the Intrinsically Disordered N-Terminal Domain of Retinoid X Receptor α in the Context of the Full-Length Protein

Belorusova A, Osz J, Petoukhov M, Peluso-Iltis C, Kieffer B, Svergun D, Rochel N
Biochemistry 55(12):1741-1748 (2016 Mar 15)

doi: 10.1021/acs.biochem.5b01122

Submitted to SASBDB: 2021 Aug 4 Published in SASBDB: 2021 Sep 10

SASDMU4 – Retinoic acid receptor RXR-alpha ligand binding domain (LBD) Sample: Retinoic acid receptor RXR-alpha dimer, 51 kDa Homo sapiens protein Buffer: 20 mM Tris, 100 mM NaCl, 100 mM KCl, 5% glycerol, 2 mM Chaps, and 5 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2010 Oct 7 RgGuinier 2.5 nm Dmax 8.3 nm VolumePorod 60 nm3

SASDMV4 – Full-length Retinoic acid receptor RXR-alpha in complex with Ramp2 DNA Sample: Retinoic acid receptor RXR-alpha dimer, 102 kDa Homo sapiens protein Ramp2 DNA monomer, 11 kDa DNA Buffer: 20 mM Tris, 50 mM NaCl, 50 mM KCl, 5% glycerol, 2 mM Chaps, and 5 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2010 May 7 RgGuinier 4.4 nm Dmax 13.9 nm VolumePorod 161 nm3

SASDMW4 – Retinoic acid receptor RXR-alpha N-terminal and DNA binding domains (DBD) in complex with Ramp2 DNA Sample: Ramp2 DNA monomer, 11 kDa DNA Retinoic acid receptor RXR-alpha dimer, 44 kDa Homo sapiens protein Buffer: 20 mM Tris, 50 mM NaCl, 50 mM KCl, 5% glycerol, 2 mM Chaps, and 5 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Jul 17 RgGuinier 4.3 nm Dmax 14.3 nm VolumePorod 89 nm3

SASDMX4 – Retinoic acid receptor RXR-alpha DNA binding (DBD) and ligand binding (LBD) domains in complex with Ramp2 DNA Sample: Ramp2 DNA monomer, 11 kDa DNA Retinoic acid receptor RXR-alpha dimer, 74 kDa Homo sapiens protein Buffer: 20 mM Tris, 50 mM NaCl, 50 mM KCl, 5% glycerol, 2 mM Chaps, and 5 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2010 Oct 7 RgGuinier 3.6 nm Dmax 12.2 nm

SASDMY4 – Retinoic acid receptor RXR-alpha DNA binding domain (DBD) in complex with Ramp2 Sample: Ramp2 DNA monomer, 11 kDa DNA Retinoic acid receptor RXR-alpha dimer, 20 kDa Homo sapiens protein Buffer: 20 mM Tris, 50 mM NaCl, 50 mM KCl, 5% glycerol, 2 mM Chaps, and 5 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2010 Oct 7 RgGuinier 1.9 nm Dmax 5.8 nm