Reaction hijacking of tyrosine tRNA synthetase as a new whole-of-life-cycle antimalarial strategy

Xie S, Metcalfe R, Dunn E, Morton C, Huang S, Puhalovich T, Du Y, Wittlin S, Nie S, Luth M, Ma L, Kim M, Pasaje C, Kumpornsin K, Giannangelo C, Houghton F, Churchyard A, Famodimu M, Barry D, Gillett D, Dey S, Kosasih C, Newman W, Niles J, Lee M, Baum J, Ottilie S, Winzeler E, Creek D, Williamson N, Parker M, Brand S, Langston S, Dick L, Griffin M, Gould A, Tilley L
Science 376(6597):1074-1079 (2022 Jun 03)

doi: 10.1126/science.abn0611 		Submitted to SASBDB: 2021 Aug 28
Published in SASBDB:

SASDMC4 – Plasmodium falciparum tyrosyl-tRNA synthetase

Tyrosine--tRNA ligase experimental SAS data
OTHER model
Sample: Tyrosine--tRNA ligase dimer, 87 kDa Plasmodium falciparum (isolate … protein
Buffer: 20 mM Tris-HCl, 150 mM NaCl, 0.5 mM TCEP, 0.1% sodium azide, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2021 Jun 4
RgGuinier 3.7 nm
Dmax 12.1 nm
VolumePorod 126 nm3

SASDMD4 – Plasmodium falciparum tyrosyl-tRNA synthetase, S234C mutant

Tyrosine--tRNA ligase (S234C) experimental SAS data
OTHER model
Sample: Tyrosine--tRNA ligase (S234C) dimer, 87 kDa Plasmodium falciparum (isolate … protein
Buffer: 20 mM Tris-HCl, 150 mM NaCl, 0.5 mM TCEP, 0.1% sodium azide, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2021 Jun 4
RgGuinier 3.7 nm
Dmax 12.0 nm
VolumePorod 124 nm3

SASDME4 – Human tyrosyl tRNA synthetase

Tyrosine--tRNA ligase, cytoplasmic experimental SAS data
OTHER model
Sample: Tyrosine--tRNA ligase, cytoplasmic dimer, 82 kDa Homo sapiens protein
Buffer: 20 mM Tris-HCl, 150 mM NaCl, 0.5 mM TCEP, 0.1% sodium azide, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2021 Jun 4
RgGuinier 3.6 nm
Dmax 11.5 nm
VolumePorod 118 nm3