Structural basis for specific RNA recognition by the alternative splicing factor RBM5.

Soni K, Jagtap PKA, Martínez-Lumbreras S, Bonnal S, Geerlof A, Stehle R, Simon B, Valcárcel J, Sattler M
Nat Commun 14(1):4233 (2023 Jul 15)
PMID: 37454201
doi: 10.1038/s41467-023-39961-w 		Submitted to SASBDB: 2021 Aug 10
Published in SASBDB:

SASDM43 – RRM1-ZnF1 tandem domains of RNA-binding protein 5 (C191G mutant)

RNA-binding protein 5 (I107T, C191G) experimental SAS data
Sample: RNA-binding protein 5 (I107T, C191G) monomer, 14 kDa Homo sapiens protein
Buffer: 20 mM MES, 400 mM NaCl, 1 mM DTT, pH: 6.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Sep 26
RgGuinier 1.7 nm
Dmax 5.6 nm
VolumePorod 28 nm3

SASDM53 – RRM1-ZnF1-RRM2 triple domains of RNA-binding protein 5 (C191G mutant)

RNA Binding Motif protein 5 (I107T, C191G) experimental SAS data
RNA Binding Motif protein 5 (I107T, C191G) Kratky plot
Sample: RNA Binding Motif protein 5 (I107T, C191G) monomer, 26 kDa Homo sapiens protein
Buffer: 20 mM MES, 400 mM NaCl, 1 mM DTT, pH: 6.5
Experiment: SAXS data collected at Rigaku BioSAXS-1000, SFB 1035, Technische Universität München on 2017 Feb 9
RgGuinier 2.3 nm
Dmax 7.8 nm
VolumePorod 36 nm3

SASDM63 – RRM1-ZnF1-RRM2 triple domains of RNA-binding protein 5 (C191G mutant) in complex with GGCU_12 RNA

RNA Binding Motif protein 5 (I107T, C191G)Caspase-2 derived RNA GGCU_12 experimental SAS data
OTHER model
Sample: RNA Binding Motif protein 5 (I107T, C191G) monomer, 26 kDa Homo sapiens protein
Caspase-2 derived RNA GGCU_12 monomer, 4 kDa RNA
Buffer: 20 mM MES, 100 mM NaCl, 1 mM DTT, pH: 6.5
Experiment: SAXS data collected at Rigaku BioSAXS-1000, SFB 1035, Technische Universität München on 2017 Mar 1
RgGuinier 2.2 nm
Dmax 7.5 nm
VolumePorod 42 nm3