Leucine Rich Repeat And Sterile Alpha Motif Containing 1 (LRSAM1)

Si Hoon Park

Submitted to SASBDB: 2021 Aug 19 Published in SASBDB: 2024 Aug 19

SASDMN3 – Wild type E3 ubiquitin-protein ligase LRSAM1 Sample: E3 ubiquitin-protein ligase LRSAM1 monomer, 84 kDa Homo sapiens protein Buffer: 25 mM Tris-HCl pH 8.0, 150 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2019 Dec 7 RgGuinier 6.1 nm Dmax 19.4 nm VolumePorod 250 nm3

SASDMP3 – Wild type E3 ubiquitin-protein ligase LRSAM1 (1-632): C-terminal PTAP/PSAP motif and RING domain deletion mutant, ΔPTAP/PSAP-RING Sample: E3 ubiquitin-protein ligase LRSAM1 - ΔPTAP/PSAP-RING monomer, 74 kDa Homo sapiens protein Buffer: 50 mM Tris-HCl pH 8.0, 300 mM NaCl, 1 mM TCEP, 5% w/v glycerol, pH: 8 Experiment: SAXS data collected at 4C, Pohang Accelerator Laboratory on 2017 Oct 24 RgGuinier 5.5 nm Dmax 18.0 nm VolumePorod 322 nm3

SASDMQ3 – Wild type E3 ubiquitin-protein ligase LRSAM1 (1-307): N-terminal LRR containing domain Sample: E3 ubiquitin-protein ligase LRSAM1 - LRR containing N-terminal domain monomer, 35 kDa Homo sapiens protein Buffer: 50 mM Tris-HCl pH 8.0, 300 mM NaCl, 1 mM TCEP, 5% w/v glycerol, pH: 8 Experiment: SAXS data collected at 4C, Pohang Accelerator Laboratory on 2017 Oct 23 RgGuinier 3.4 nm Dmax 8.2 nm VolumePorod 105 nm3

SASDMR3 – Wild type E3 ubiquitin-protein ligase LRSAM1 (561-632): SAM domain Sample: E3 ubiquitin-protein ligase LRSAM1 - SAM domain monomer, 8 kDa Homo sapiens protein Buffer: 50 mM Tris-HCl pH 8.0, 150 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at 4C, Pohang Accelerator Laboratory on 2019 Apr 4 RgGuinier 1.4 nm Dmax 4.3 nm VolumePorod 11 nm3

SASDMS3 – Wild type E3 ubiquitin-protein ligase LRSAM1 (80-171): domains LRR3-6 in the Repebody scaffold Sample: E3 ubiquitin-protein ligase LRSAM1 monomer, 30 kDa Homo sapiens protein Buffer: 50 mM Tris-HCl pH 8.0, 150 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at 4C, Pohang Accelerator Laboratory on 2019 Apr 4 RgGuinier 2.5 nm Dmax 8.3 nm VolumePorod 43 nm3