Solution structure of human Pex5.Pex14.PTS1 protein complexes obtained by small angle X-ray scattering.

Shiozawa K, Konarev PV, Neufeld C, Wilmanns M, Svergun DI
J Biol Chem 284(37):25334-42 (2009 Sep 11)

PMID: 19584060 doi: 10.1074/jbc.M109.002311

Submitted to SASBDB: 2021 Aug 19 Published in SASBDB: 2023 Oct 10

SASDN33 – Ternary Human Pex5p(C-terminal)-Pex14p(N)-PTS1 Complex (1:1:1 stoichiometry) Sample: Peroxisomal targeting signal 1 receptor (C -terminal) monomer, 48 kDa Homo sapiens protein Peroxisomal membrane protein PEX14 (N-terminal) monomer, 7 kDa Homo sapiens protein PTS1-BP monomer, 14 kDa Homo sapiens protein Buffer: 50 mM HEPES-KOH (pH 7.5), 100mM KCl, and 20mM TCEP, pH: 7.5 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2005 Oct 3 RgGuinier 2.9 nm Dmax 9.0 nm VolumePorod 110 nm3

SASDN43 – Full-length Human Pex5p protein Sample: Peroxisomal targeting signal 1 receptor monomer, 71 kDa Homo sapiens protein Buffer: 50 mM HEPES-KOH (pH 7.5), 100mM KCl, and 20mM TCEP, pH: 7.5 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Feb 24 RgGuinier 5.0 nm Dmax 20.0 nm VolumePorod 181 nm3

SASDN53 – Ternary Human Pex5p(full-length)-Pex14p(N)-PTS1 Protein Complex (1:7:1 stoichiometry) Sample: Peroxisomal targeting signal 1 receptor monomer, 71 kDa Homo sapiens protein Peroxisomal membrane protein PEX14 monomer, 7 kDa Homo sapiens protein PTS1-BP monomer, 13 kDa Homo sapiens protein Buffer: 50 mM HEPES-KOH (pH 7.5), 100mM KCl, and 20mM TCEP, pH: 7.5 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Mar 18 RgGuinier 6.0 nm Dmax 20.0 nm VolumePorod 267 nm3