The Cytoplasmic Tail of Influenza A Virus Hemagglutinin and Membrane Lipid Composition Change the Mode of M1 Protein Association with the Lipid Bilayer

Kordyukova L, Konarev P, Fedorova N, Shtykova E, Ksenofontov A, Loshkarev N, Dadinova L, Timofeeva T, Abramchuk S, Moiseenko A, Baratova L, Svergun D, Batishchev O
Membranes 11(10):772 (2021 Oct 10)

doi: 10.3390/membranes11100772

Submitted to SASBDB: 2021 Aug 20 Published in SASBDB: 2021 Oct 14

SASDMF5 – Two-component synthetic liposomes composed of 10% DOPS + 90% DOPC lipid mixtures loaded with M1 protein Sample: Two-component synthetic liposomes composed of 10% DOPS + 90% DOPC lipid mixtures loaded with M1 protein ((lipid:M1 molar ratio 20:1) monomer, 2000 kDa Buffer: 100 mM NaCl, 50 mM MES buffer, pH: 6.8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Oct 30

SASDMG5 – Two-component synthetic liposomes composed of 30% DOPS + 70% DOPC lipid mixtures loaded with M1 protein Sample: Two-component synthetic liposomes composed of 30% DOPS + 70% DOPC lipid mixtures loaded with M1 protein (lipid:M1 molar ration 20:1) monomer, 2000 kDa Buffer: 100 mM NaCl, 50 mM MES buffer, pH: 6.8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Oct 31

SASDMH5 – Four-component synthetic liposomes composed of 30% bPS + 10% POPC + 40% SM + 20% Chol lipid mixtures loaded with M1 protein Sample: Four-component synthetic liposomes composed of 30% bPS + 10% POPC + 40% SM + 20% Chol lipid mixtures loaded with M1 protein (lipid:M1 molar ration 20:1) monomer, 2000 kDa Buffer: 100 mM NaCl, 50 mM MES buffer, pH: 6.8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Oct 31

SASDMJ5 – Four-component synthetic liposomes composed of 20% bPS + 13.3% POPC + 33.3% SM + 33.3% Chol lipid mixtures loaded with M1 protein Sample: Four-component synthetic liposomes composed of 20% bPS + 13.3% POPC + 33.3% SM + 33.3% Chol lipid mixtures loaded with M1 protein (lipid:M1 molar ration 20:1) monomer, 2000 kDa Buffer: 100 mM NaCl, 50 mM MES buffer, pH: 6.8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Oct 31

SASDMK5 – Native liposomes composed of lipids extracted from A/Puerto Rico/8/34 (H1N1) virus envelope loaded with M1 protein. Sample: Native liposomes composed of lipids extracted from A/Puerto Rico/8/34 (H1N1) virus envelope loaded with M1 protein. (lipid:M1 molar ration 4:1) monomer, 2000 kDa Buffer: 100 mM NaCl, 50 mM MES buffer, pH: 6.8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Nov 26

SASDML5 – Proteoliposomes composed of lipids from A/Puerto Rico/8/34 (H1N1) virus envelope together with the HA LI45 peptides loaded with M1 protein Sample: Proteoliposomes composed of lipids from A/Puerto Rico/8/34 (H1N1) virus envelope together with the HA LI45 peptides loaded with M1 protein (lipid:M1 molar ration 4:1) monomer, 2000 kDa Buffer: 100 mM NaCl, 50 mM MES buffer, pH: 6.8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Nov 26