Hybrid Structural Analysis of the Arp2/3 Regulator Arpin Identifies Its Acidic Tail as a Primary Binding Epitope.

Fetics S, Thureau A, Campanacci V, Aumont-Nicaise M, Dang I, Gautreau A, Pérez J, Cherfils J
Structure 24(2):252-60 (2016 Feb 2)

PMID: 26774128 doi: 10.1016/j.str.2015.12.001

Submitted to SASBDB: 2015 Dec 3 Published in SASBDB: 2016 Jul 3

SASDBT2 – Ankyrin repeat domains from human Tankyrase-2 (489-649) Sample: Ankyrin repeat domains from Tankyrase 2 monomer, 18 kDa Homo sapiens protein Buffer: 50 mM HEPES 100mM NaCl 1mM TCEP, pH: 7.5 Experiment: SAXS data collected at SWING, SOLEIL on 2014 Dec 4 RgGuinier 1.8 nm Dmax 6.3 nm VolumePorod 24 nm3

SASDBU2 – Human Arpin (isoform 1) Sample: Human Arpin monomer, 25 kDa Homo sapiens protein Buffer: 50 mM HEPES 100mM NaCl 1mM TCEP, pH: 7.5 Experiment: SAXS data collected at SWING, SOLEIL on 2014 Dec 4 RgGuinier 2.6 nm Dmax 13.2 nm VolumePorod 47 nm3

SASDBV2 – Zebrafish Arpin Sample: Zebrafish (Danio rerio) Arpin monomer, 25 kDa Danio rerio protein Buffer: 50 mM HEPES 100mM NaCl 1mM TCEP, pH: 7.5 Experiment: SAXS data collected at SWING, SOLEIL on 2014 Dec 4 RgGuinier 2.7 nm Dmax 13.8 nm VolumePorod 47 nm3

SASDBW2 – Zebrafish (Danio rerio) Arpin truncated C-terminal mutant (delta-C 16). Sample: Zebrafish (Danio rerio) Arpin truncated C-terminal mutant (delta-C 16). monomer, 24 kDa Danio rerio protein Buffer: 50 mM HEPES 100mM NaCl 1mM TCEP, pH: 7.5 Experiment: SAXS data collected at SWING, SOLEIL on 2014 Dec 4 RgGuinier 2.2 nm Dmax 11.3 nm VolumePorod 37 nm3

SASDBX2 – Zebrafish Arpin in complex with the ankyrin repeat domains of human Tankyrase 2 (489-469) Sample: Zebrafish arpin/human tankyrase 2 ankyrin repeat domain complex monomer, 43 kDa Danio rerio / … protein Buffer: 50 mM HEPES 100mM NaCl 1mM TCEP, pH: 7.5 Experiment: SAXS data collected at SWING, SOLEIL on 2014 Dec 4 RgGuinier 3.3 nm Dmax 12.6 nm VolumePorod 58 nm3