Dependence of the Nanoscale Composite Morphology of Fe3O4 Nanoparticle-Infused Lysozyme Amyloid Fibrils on Timing of Infusion: A Combined SAXS and AFM Study

Schroer M, Hu P, Tomasovicova N, Batkova M, Zakutanska K, Wu P, Kopcansky P
Molecules 26(16):4864 (2021 Aug 11)

doi: 10.3390/molecules26164864

Submitted to SASBDB: 2021 Sep 2 Published in SASBDB: 2021 Sep 3

SASDMY3 – Lysozyme amyloid fibril (LAF) Sample: Lysozyme amyloid fibril, 1 kDa Gallus gallus protein Buffer: 0.2 M glycine-HCl, 80 mM NaCl, pH: 2.2 Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Nov 29 RgGuinier 38.4 nm Dmax 80.0 nm

SASDMZ3 – Fe3O4 nanoparticles (10 nm diameter) Sample: Fe3O4 nanoparticles; nominal diameter 10 nm (hydrodynamic diameter) monomer, 1 kDa Buffer: 50 mM borate buffer, 0.02% NaN3, pH: 8.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Nov 29 RgGuinier 7.0 nm Dmax 8.0 nm

SASDM24 – Fe3O4 nanoparticles (20 nm diameter) Sample: Fe3O4 nanoparticles; nominal diameter 20 nm (hydrodynamic diameter) monomer, 1 kDa Buffer: 50 mM borate buffer, 0.02% NaN3, pH: 8.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Nov 29 RgGuinier 11.0 nm Dmax 14.0 nm

SASDM34 – Fe3O4 nanoparticles (30 nm diameter) Sample: Fe3O4 nanoparticles; nominal diameter 30 nm (hydrodynamic diameter) monomer, 1 kDa Buffer: 50 mM borate buffer, 0.02% NaN3, pH: 8.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Nov 29 RgGuinier 18.1 nm Dmax 16.9 nm

SASDM44 – LAF + P10 nanocomposite (NP added before fibrilization) Sample: Lysozyme amyloid fibril, 1 kDa Gallus gallus protein Fe3O4 nanoparticles; nominal diameter 10 nm (hydrodynamic diameter) monomer, 1 kDa Buffer: 0.2 M glycine-HCl, 80 mM NaCl, pH: 2.2 Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Nov 29 RgGuinier 22.3 nm Dmax 70.0 nm

SASDM54 – LAF + P30 nanocomposite (NP added before fibrilization) Sample: Lysozyme amyloid fibril, 1 kDa Gallus gallus protein Fe3O4 nanoparticles; nominal diameter 30 nm (hydrodynamic diameter) monomer, 1 kDa Buffer: 0.2 M glycine-HCl, 80 mM NaCl, pH: 2.2 Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Nov 29 RgGuinier 22.5 nm Dmax 95.0 nm

SASDM64 – LAF + P20 nanocomposite (NP added before fibrilization) Sample: Lysozyme amyloid fibril, 1 kDa Gallus gallus protein Fe3O4 nanoparticles; nominal diameter 20 nm (hydrodynamic diameter) monomer, 1 kDa Buffer: 0.2 M glycine-HCl, 80 mM NaCl, pH: 2.2 Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Nov 29 RgGuinier 23.6 nm Dmax 90.0 nm

SASDM74 – LAF + P20 nanocomposite (NP added after fibrilization) Sample: Lysozyme amyloid fibril, 1 kDa Gallus gallus protein Fe3O4 nanoparticles; nominal diameter 20 nm (hydrodynamic diameter) monomer, 1 kDa Buffer: 0.2 M glycine-HCl, 80 mM NaCl, pH: 2.2 Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Sep 2 RgGuinier 31.0 nm Dmax 75.0 nm