Pathogen-specific structural features of Candida albicans Ras1 activation complex: uncovering new antifungal drug targets.

Manso JA, Carabias A, Sárkány Z, de Pereda JM, Pereira PJB, Macedo-Ribeiro S
mBio :e0063823 (2023 Aug 1)
PMID: 37526476
doi: 10.1128/mbio.00638-23 		Submitted to SASBDB: 2021 Sep 10
Published in SASBDB:

SASDM55 – GTP-binding domain of Candida albicans Ras-like protein 1

GTP-binding domain of Ras-like protein 1 experimental SAS data
MODELLER model
Sample: GTP-binding domain of Ras-like protein 1 monomer, 19 kDa protein
Buffer: 20 mM Tris-HCl, 150 mM NaCl, 5% glycerol, 3 mM DTT, 5 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 21
RgGuinier 1.6 nm
Dmax 4.1 nm
VolumePorod 26 nm3

SASDM65 – Candida albicans Ras-like protein 1

Ras-like protein 1 experimental SAS data
Candida albicans Ras-like protein 1 Rg histogram
Sample: Ras-like protein 1 monomer, 33 kDa Candida albicans protein
Buffer: 20 mM Tris-HCl, 150 mM NaCl, 5% glycerol, 3 mM DTT, 5 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 21
RgGuinier 3.6 nm
Dmax 15.1 nm
VolumePorod 56 nm3

SASDM75 – Ras-like protein 1 guanine nucleotide exchange factor region of Candida albicans cell division control protein 25

Cell division control protein 25 experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Cell division control protein 25 monomer, 55 kDa Candida albicans (strain … protein
Buffer: 20 mM Na-phosphate, 150 mM NaCl, 5% glycerol, 3 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 21
RgGuinier 3.2 nm
Dmax 11.8 nm
VolumePorod 81 nm3

SASDM85 – GTP-binding domain of Candida albicans Ras-like protein 1 in complex with the guanine nucleotide exchange factor region of cell division control protein 25

GTP-binding domain of Ras-like protein 1Cell division control protein 25 experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: GTP-binding domain of Ras-like protein 1 monomer, 19 kDa protein
Cell division control protein 25 monomer, 55 kDa Candida albicans (strain … protein
Buffer: 20 mM Na-phosphate, 150 mM NaCl, 1 mM EDTA, 5% glycerol, 3 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 21
RgGuinier 3.2 nm
Dmax 11.8 nm
VolumePorod 99 nm3

SASDM95 – Candida albicans Ras-like protein 1 in complex with the guanine nucleotide exchange factor region of cell division control protein 25

Ras-like protein 1Cell division control protein 25 experimental SAS data
Candida albicans Ras-like protein 1 in complex with the guanine nucleotide exchange factor region of cell division control protein 25 Rg histogram
Sample: Ras-like protein 1 monomer, 33 kDa Candida albicans protein
Cell division control protein 25 monomer, 55 kDa Candida albicans (strain … protein
Buffer: 20 mM Na-phosphate, 150 mM NaCl, 1 mM EDTA, 5% glycerol, 3 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 21
RgGuinier 4.0 nm
Dmax 17.7 nm
VolumePorod 149 nm3