The two-step cargo recognition mechanism of heterotrimeric kinesin.

Jiang X, Ogawa T, Yonezawa K, Shimizu N, Ichinose S, Uchihashi T, Nagaike W, Moriya T, Adachi N, Kawasaki M, Dohmae N, Senda T, Hirokawa N
EMBO Rep :e56864 (2023 Aug 14)

PMID: 37575008 doi: 10.15252/embr.202356864

Submitted to SASBDB: 2021 Oct 8 Published in SASBDB: 2023 Aug 7

SASDMV5 – Kinesin superfamily protein 3 A/B and kinesin associated protein 3 (KAP) Sample: Kinesin-like protein KIF3B monomer, 32 kDa Mus musculus protein Kinesin-associated protein 3 monomer, 81 kDa Mus musculus protein Kinesin-like protein KIF3A monomer, 28 kDa Mus musculus protein Buffer: 20 mM Tris-HCl, 200 mM NaCl, 5% glycerol, 1 mM DTT, pH: 8 Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2020 Jun 24 RgGuinier 5.8 nm Dmax 27.0 nm VolumePorod 557 nm3

SASDMW5 – Kinesin superfamily protein 3 A/B and kinesin associated protein 3 (KAP) with APC ARM Sample: Adenomatous polyposis coli protein (N-terminal ARM domain) monomer, 75 kDa Mus musculus protein Kinesin-like protein KIF3A monomer, 28 kDa Mus musculus protein Kinesin-like protein KIF3B monomer, 32 kDa Mus musculus protein Kinesin-associated protein 3 monomer, 81 kDa Mus musculus protein Buffer: 25 mM Tris-HCl, 200 mM NaCl, 1 mM DTT, 5% glycerol, pH: 8 Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2020 Nov 17 RgGuinier 5.4 nm Dmax 19.5 nm VolumePorod 763 nm3