Structural basis of dimerization and nucleic acid binding of human DBHS proteins NONO and PSPC1.

Knott GJ, Chong YS, Passon DM, Liang XH, Deplazes E, Conte MR, Marshall AC, Lee M, Fox AH, Bond CS
Nucleic Acids Res (2021 Dec 14)

PMID: 34904671 doi: 10.1093/nar/gkab1216

Submitted to SASBDB: 2021 Oct 15 Published in SASBDB: 2021 Dec 20

SASDMR6 – 5-10-5 gapmer phosphorothioate antisense oligonucleotide Sample: 5-10-5 gapmer phosphorothioate antisense oligonucleotide dimer, 14 kDa Buffer: 20 mM Tris-Cl (pH 7.5), 250 mM KCl, 50 mM L-proline, 0.5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2015 Apr 28 RgGuinier 1.8 nm Dmax 8.5 nm VolumePorod 15 nm3

SASDMS6 – Non-POU domain-containing octamer-binding protein (NONO homodimer) Sample: Non-POU domain-containing octamer-binding protein dimer, 60 kDa Homo sapiens protein Buffer: 20 mM Tris-Cl (pH 7.5), 250 mM KCl, 50 mM L-proline, 0.5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2015 Apr 28 RgGuinier 2.8 nm Dmax 9.5 nm VolumePorod 96 nm3

SASDMT6 – Non-POU domain-containing octamer-binding protein (NONO homodimer) bound to an antisense oligonucleotide Sample: Non-POU domain-containing octamer-binding protein dimer, 60 kDa Homo sapiens protein 5-10-5 gapmer phosphorothioate antisense oligonucleotide tetramer tetramer, 28 kDa Buffer: 20 mM Tris-Cl (pH 7.5), 250 mM KCl, 50 mM L-proline, 0.5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2015 Apr 28 RgGuinier 3.9 nm Dmax 18.4 nm VolumePorod 153 nm3