The crystal structure of the EspB-EspK virulence factor-chaperone complex suggests an additional type VII secretion mechanism in M. tuberculosis.

Gijsbers A, Eymery M, Gao Y, Menart I, Vinciauskaite V, Siliqi D, Peters PJ, McCarthy A, Ravelli RBG
J Biol Chem :102761 (2022 Dec 1)

PMID: 36463964 doi: 10.1016/j.jbc.2022.102761

Submitted to SASBDB: 2021 Oct 20 Published in SASBDB: 2022 Dec 12

SASDMD7 – ESX-1 secretion-associated protein EspB large construct Sample: ESX-1 secretion-associated protein EspB monomer, 48 kDa Mycobacterium tuberculosis (strain … protein Buffer: 20 mM Tris-HCl, 300 mM NaCl, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Sep 21 RgGuinier 5.0 nm Dmax 19.2 nm VolumePorod 119 nm3

SASDME7 – ESX-1 secretion-associated protein EspB medium construct Sample: ESX-1 secretion-associated protein EspB monomer, 37 kDa Mycobacterium tuberculosis (strain … protein Buffer: 20 mM Tris-HCl, 300 mM NaCl, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Sep 21 RgGuinier 3.5 nm Dmax 13.3 nm VolumePorod 75 nm3

SASDQF4 – ESX-1 secretion-associated protein EspB Sample: ESX-1 secretion-associated protein EspB heptamer, 261 kDa Mycobacterium tuberculosis (strain … protein Buffer: 20 mM Tris-HCl, 300 mM NaCl, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Sep 12 RgGuinier 5.9 nm Dmax 18.8 nm VolumePorod 911 nm3

SASDMF7 – ESX-1 secretion-associated protein EspB small construct Sample: ESX-1 secretion-associated protein EspB monomer, 30 kDa Mycobacterium tuberculosis (strain … protein Buffer: 20 mM Tris-HCl, 300 mM NaCl, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Sep 21 RgGuinier 3.3 nm Dmax 10.8 nm VolumePorod 56 nm3

SASDMG7 – ESX-1 secretion-associated protein EspB large construct bound to ESX-1 secretion-associated protein EspK — EspBL-K complex Sample: ESX-1 secretion-associated protein EspK monomer, 27 kDa Mycobacterium tuberculosis (strain … protein ESX-1 secretion-associated protein EspB monomer, 48 kDa Mycobacterium tuberculosis (strain … protein Buffer: 20 mM Tris-HCl, 300 mM NaCl, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Apr 12 RgGuinier 5.0 nm Dmax 18.3 nm VolumePorod 128 nm3

SASDMH7 – ESX-1 secretion-associated protein EspB medium construct bound to ESX-1 secretion-associated protein EspK — EspBM-K complex Sample: ESX-1 secretion-associated protein EspK monomer, 27 kDa Mycobacterium tuberculosis (strain … protein ESX-1 secretion-associated protein EspB monomer, 37 kDa Mycobacterium tuberculosis (strain … protein Buffer: 20 mM Tris-HCl, 300 mM NaCl, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Apr 12 RgGuinier 4.3 nm Dmax 15.7 nm VolumePorod 100 nm3

SASDMJ7 – ESX-1 secretion-associated protein EspB small construct bound to ESX-1 secretion-associated protein EspK — EspBS-K complex Sample: ESX-1 secretion-associated protein EspK monomer, 27 kDa Mycobacterium tuberculosis (strain … protein ESX-1 secretion-associated protein EspB monomer, 30 kDa Mycobacterium tuberculosis (strain … protein Buffer: 20 mM Tris-HCl, 300 mM NaCl, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Sep 12 RgGuinier 4.1 nm Dmax 13.0 nm VolumePorod 113 nm3