Sas20 is a highly flexible starch-binding protein in the Ruminococcus bromii cell-surface amylosome

Cerqueira F, Photenhauer A, Doden H, Brown A, Abdel-Hamid A, Moraïs S, Bayer E, Wawrzak Z, Cann I, Ridlon J, Hopkins J, Koropatkin N
Journal of Biological Chemistry :101896 (2022 Apr)

doi: 10.1016/j.jbc.2022.101896

Submitted to SASBDB: 2021 Dec 6 Published in SASBDB: 2022 Apr 4

SASDMZ9 – Dockerin domain-containing protein, starch adherence system 20 (Sas20), domains 1 and 2 Sample: Dockerin domain-containing protein monomer, 57 kDa Ruminococcus bromii protein Buffer: phosphate buffered saline, 1 mM TCEP, pH: 7 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Mar 28 RgGuinier 5.4 nm Dmax 20.3 nm VolumePorod 93 nm3

SASDN22 – Dockerin domain-containing protein, starch adherence system 20 (Sas20), domain 1 with maltoheptaose Sample: Dockerin domain-containing protein monomer, 26 kDa Ruminococcus bromii protein Buffer: phosphate buffered saline, 1 mM TCEP, pH: 7 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 14 RgGuinier 2.0 nm Dmax 7.8 nm VolumePorod 45 nm3

SASDN32 – Dockerin domain-containing protein, starch adherence system 20 (Sas20), domain 2 with maltoheptaose Sample: Dockerin domain-containing protein monomer, 26 kDa Ruminococcus bromii protein Buffer: phosphate buffered saline, 1 mM TCEP, pH: 7 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 14 RgGuinier 2.1 nm Dmax 7.4 nm VolumePorod 41 nm3

SASDN42 – Dockerin domain-containing protein, starch adherence system 20 (Sas20), domains 1 and 2 with maltoheptaose Sample: Dockerin domain-containing protein monomer, 26 kDa Ruminococcus bromii protein Buffer: phosphate buffered saline, 1 mM TCEP, pH: 7 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 14 RgGuinier 5.2 nm Dmax 19.0 nm VolumePorod 84 nm3

SASDMX9 – Dockerin domain-containing protein, starch adherence system 20 (Sas20), domain 1 Sample: Dockerin domain-containing protein monomer, 26 kDa Ruminococcus bromii protein Buffer: phosphate buffered saline, 1 mM TCEP, pH: 7 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Mar 28 RgGuinier 2.1 nm Dmax 10.3 nm VolumePorod 42 nm3

SASDMY9 – Dockerin domain-containing protein, starch adherence system 20 (Sas20), domain 2 Sample: Dockerin domain-containing protein monomer, 26 kDa Ruminococcus bromii protein Buffer: phosphate buffered saline, 1 mM TCEP, pH: 7 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Mar 28 RgGuinier 2.3 nm Dmax 7.8 nm VolumePorod 35 nm3