Structural basis of the zinc-induced cytoplasmic aggregation of the RNA-binding protein SFPQ

Huang J, Ringuet M, Whitten A, Caria S, Lim Y, Badhan R, Anggono V, Lee M
Nucleic Acids Research 48(6):3356-3365 (2020 Feb 08)

doi: 10.1093/nar/gkaa076 		Submitted to SASBDB: 2021 Nov 5
Published in SASBDB:

SASDMG8 – Splicing factor, proline- and glutamine-rich-QM bound to Non-POU domain-containing octamer-binding protein: SFPQ(276-598)-QM / NONO(53-312) heterodimer

Non-POU domain-containing octamer-binding proteinSplicing factor, proline- and glutamine-rich experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Non-POU domain-containing octamer-binding protein monomer, 30 kDa Homo sapiens protein
Splicing factor, proline- and glutamine-rich monomer, 38 kDa Homo sapiens protein
Buffer: 20 mM Tris, 250 mM NaCl, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2018 Apr 5
RgGuinier 4.0 nm
Dmax 16.5 nm
VolumePorod 90 nm3

SASDMH8 – Splicing factor, proline- and glutamine-rich-QM-TM bound to Non-POU domain-containing octamer-binding protein: SFPQ(276-598)-QM-TM / NONO(52-312) heterodimer

Splicing factor, proline- and glutamine-richNon-POU domain-containing octamer-binding protein experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Splicing factor, proline- and glutamine-rich monomer, 38 kDa Homo sapiens protein
Non-POU domain-containing octamer-binding protein monomer, 30 kDa Homo sapiens protein
Buffer: 20 mM Tris, 250 mM NaCl, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2018 Nov 8
RgGuinier 3.6 nm
Dmax 16.5 nm
VolumePorod 80 nm3