Structural plasticity of the coiled-coil interactions in human SFPQ.

Koning HJ, Lai JY, Marshall AC, Stroeher E, Monahan G, Pullakhandam A, Knott GJ, Ryan TM, Fox AH, Whitten A, Lee M, Bond CS
Nucleic Acids Res (2024 Dec 19)

PMID: 39698821 doi: 10.1093/nar/gkae1198

Submitted to SASBDB: 2021 Nov 9 Published in SASBDB: 2023 Nov 9

SASDMV7 – The tetramer of splicing factor, proline- and glutamine-rich and Non-POU domain-containing octamer-binding protein (SFPQ214-598(R542C)/NONO53-312) Sample: Splicing factor, proline- and glutamine-rich dimer, 153 kDa Homo sapiens protein Non-POU domain-containing octamer-binding protein dimer, 60 kDa Homo sapiens protein Buffer: 20 mM Tris pH 7.5, 250 mM NaCl, pH: 7.5 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Jun 18 RgGuinier 5.5 nm Dmax 20.4 nm VolumePorod 304 nm3

SASDMW8 – The disulphide-linked tetramer of splicing factor, proline- and glutamine-rich and Non-POU domain-containing octamer-binding protein (SFPQ276-598(R542C)/NONO53-312) at 0.78mg/ml Sample: Non-POU domain-containing octamer-binding protein dimer, 60 kDa Homo sapiens protein Splicing factor, proline- and glutamine-rich SFPQ276-598(R542C)/NONO53-312 dimer) dimer, 76 kDa Homo sapiens protein Buffer: 20 mM Tris pH 7.5, 250 mM NaCl, pH: 7.5 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2015 Apr 28 RgGuinier 5.2 nm Dmax 21.0 nm VolumePorod 257 nm3

SASDMW7 – Concentration dependent dimer-tetramer transition of splicing factor, proline- and glutamine-rich with Non-POU domain-containing octamer-binding protein (SFPQ276-565/NONO53-312) Sample: Splicing factor, proline- and glutamine-rich (276-565) monomer, 34 kDa Homo sapiens protein Non-POU domain-containing octamer-binding protein (53-312) monomer, 30 kDa Homo sapiens protein Buffer: 20 mM Tris pH 7.5, 250 mM NaCl, pH: 7.5 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2015 Apr 28 RgGuinier 3.0 nm Dmax 12.7 nm VolumePorod 102 nm3