Dependence of concentration of precursor clusters formed in lysozyme crystallization solutions on degree of supersaturation and its effect on character of solution transition from liquid to condensed phase

Petr Konarev Submitted to SASBDB: 2021 Nov 9
Published in SASBDB:

SASDM79 – Lysozyme crystallization solutions with precipitants from crystallization kits CS 1 and CS2 (mixture of monomers, dimers and octamers, with octamer volume fractions from 0 to 0.7%)

Lysozyme C experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Lysozyme C monomer, 14 kDa Gallus gallus protein
Buffer: 100 mM HEPES pH 7.5, 20 %(v/v) jeffamine M-600, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Aug 28
RgGuinier 1.8 nm

SASDM89 – Lysozyme crystallization solutions with precipitants from crystallization kits CS 1 and CS2 (mixture of monomers, dimers and octamers, with octamer volume fractions from 0.9% to 4.4%)

Lysozyme C experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Lysozyme C monomer, 14 kDa Gallus gallus protein
Buffer: 100 mM sodium acetate, pH 4.6, 2.0 M sodium formate, pH: 4.6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Aug 28
RgGuinier 2.2 nm

SASDM99 – Lysozyme crystallization solutions with precipitants from crystallization kits CS 1 and CS2 (mixture of monomers, dimers and octamers, with octamer volume fractions from 4.9% to 21.1%)

Lysozyme C experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Lysozyme C monomer, 14 kDa Gallus gallus protein
Buffer: 200 mM K/Na tartrate, 100 mM tri-sodium citrate pH 5.6, 2.0 M ammonium sulfate, pH: 5.6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Aug 28
RgGuinier 2.4 nm