Structural, Thermodynamic and Enzymatic Characterization of N,N-Diacetylchitobiose Deacetylase from Pyrococcus chitonophagus.

Biniek-Antosiak K, Bejger M, Śliwiak J, Baranowski D, Mohammed ASA, Svergun DI, Rypniewski W
Int J Mol Sci 23(24) (2022 Dec 12)
PMID: 36555375
doi: 10.3390/ijms232415736 		Submitted to SASBDB: 2021 Nov 24
Published in SASBDB:

SASDMA9 – Diacetylchitobiose deacetylase (isolated hexamer from SEC-SAXS)

Diacetylchitobiose deacetylase experimental SAS data
PYMOL model
Sample: Diacetylchitobiose deacetylase hexamer, 186 kDa Thermococcus chitonophagus protein
Buffer: 20 mM TRIS, 200 mM NaCl, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Sep 26
RgGuinier 3.6 nm
Dmax 11.2 nm
VolumePorod 317 nm3

SASDMB9 – Diacetylchitobiose deacetylase (oligomeric mixture of hexamers and dodecamers)

Diacetylchitobiose deacetylase experimental SAS data
PYMOL model
Sample: Diacetylchitobiose deacetylase, 185 kDa Thermococcus chitonophagus protein
Buffer: 20 mM TRIS, 200 mM NaCl, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Sep 26
RgGuinier 3.9 nm