A structure-based mechanism for displacement of the HEXIM adapter from 7SK small nuclear RNA.

Pham VV, Gao M, Meagher JL, Smith JL, D'Souza VM
Commun Biol 5(1):819 (2022 Aug 15)

PMID: 35970937 doi: 10.1038/s42003-022-03734-w

Submitted to SASBDB: 2021 Dec 2 Published in SASBDB: 2022 Aug 17

SASDME9 – 7SK stem-loop 1 with HEXIM Arginine Rich Motif Sample: Homo sapiens RNA component of 7SK nuclear ribonucleoprotein (RN7SK), small nuclear RNA monomer, 18 kDa Homo sapiens RNA Protein HEXIM1 monomer, 2 kDa Homo sapiens protein Buffer: 10 mM phosphate, 70 mM NaCl, 0.1 mM EDTA, pH: 5.6 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Nov 27 RgGuinier 2.2 nm Dmax 8.2 nm VolumePorod 26 nm3

SASDMF9 – 7SK small nuclear RNA stem loop-1 bound to the arginine rich motif of HIV-1 Tat Group M, Subtype B (Isolate A18188) Sample: Protein Tat monomer, 2 kDa Human immunodeficiency virus … protein Homo sapiens RNA component of 7SK nuclear ribonucleoprotein (RN7SK), small nuclear RNA monomer, 18 kDa Homo sapiens RNA Buffer: 10 mM phosphate, 70 mM NaCl, 0.1 mM EDTA, pH: 5.6 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Sep 9 RgGuinier 2.3 nm Dmax 9.7 nm VolumePorod 28 nm3

SASDMD9 – 7SK small nuclear RNA stem loop-1 bound to the arginine rich motif of HIV-1 Tat Group M, Subtype G Sample: Homo sapiens RNA component of 7SK nuclear ribonucleoprotein (RN7SK), small nuclear RNA monomer, 18 kDa Homo sapiens RNA Protein Tat monomer, 2 kDa Human immunodeficiency virus … protein Buffer: 10 mM phosphate, 70 mM NaCl, 0.1 mM EDTA, pH: 5.6 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Nov 27 RgGuinier 2.5 nm Dmax 9.9 nm VolumePorod 28 nm3