Ca 2+ ‐induced structural changes and intramolecular interactions in N‐terminal region of diacylglycerol kinase alpha

Takahashi D, Yonezawa K, Okizaki Y, Caaveiro J, Ueda T, Shimada A, Sakane F, Shimizu N
Protein Science 31(7) (2022 Jun 16)

doi: 10.1002/pro.4365 		Submitted to SASBDB: 2022 Jan 17
Published in SASBDB:

SASDN55 – A recoverin homology domain with EF hand domain of diacylglycerol kinase alpha under the condition with CaCl2

Diacylglycerol kinase alpha experimental SAS data
DAMMIF model
Sample: Diacylglycerol kinase alpha monomer, 22 kDa Homo sapiens protein
Buffer: 20 mM TrisHCl, 200 mM NaCl, 5 mM DTT, 5% v/v glycerol, 3 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2020 Dec 8
RgGuinier 1.9 nm
Dmax 6.2 nm
VolumePorod 44 nm3

SASDN65 – A recoverin homology domain with EF hand domain of diacylglycerol kinase alpha under the condition without CaCl2

Diacylglycerol kinase alpha experimental SAS data
DAMMIF model
Sample: Diacylglycerol kinase alpha monomer, 22 kDa Homo sapiens protein
Buffer: 20mM TrisHCl, 200mM NaCl, 5mM DTT, 5% v/v Glycerol, 3mM EGTA, pH: 7.4
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2020 Dec 8
RgGuinier 1.9 nm
Dmax 7.0 nm
VolumePorod 44 nm3