Relative domain orientation of the L289K HIV ‐1 reverse transcriptase monomer

Xi Z, Ilina T, Guerrero M, Fan L, Sluis‐Cremer N, Wang Y, Ishima R
Protein Science 31(5) (2022 Apr 11)

doi: 10.1002/pro.4307

Submitted to SASBDB: 2021 Dec 14 Published in SASBDB: 2022 Apr 11

SASDNH3 – HIV-1 reverse transcriptase L289K mutation at 1 mg/mL Sample: Gag-Pol polyprotein (HIV-1 reverse transcriptase C38V/C280S, L289K truncated to 1 - 556) monomer, 65 kDa Human immunodeficiency virus … protein Buffer: 50 mM Bis-Tris pH 6.8, 100 mM NaCl, 1% glycerol, 0.02% w/v NaN3, pH: 6.8 Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Jun 20 RgGuinier 3.2 nm Dmax 10.7 nm VolumePorod 91 nm3

SASDNJ3 – HIV-1 reverse transcriptase L289K mutation at 2 mg/mL Sample: Gag-Pol polyprotein (HIV-1 reverse transcriptase C38V/C280S, L289K truncated to 1 - 556) monomer, 65 kDa Human immunodeficiency virus … protein Buffer: 50 mM Bis-Tris pH 6.8, 100 mM NaCl, 1% glycerol, 0.02% w/v NaN3, pH: 6.8 Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Jun 20 RgGuinier 3.2 nm Dmax 10.8 nm VolumePorod 91 nm3

SASDNK3 – HIV-1 reverse transcriptase L289K mutation at 4.3 mg/mL Sample: Gag-Pol polyprotein (HIV-1 reverse transcriptase C38V/C280S, L289K truncated to 1 - 556) monomer, 65 kDa Human immunodeficiency virus … protein Buffer: 50 mM Bis-Tris pH 6.8, 100 mM NaCl, 1% glycerol, 0.02% w/v NaN3, pH: 6.8 Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Jun 20 RgGuinier 3.2 nm Dmax 10.7 nm VolumePorod 93 nm3

SASDNL3 – HIV-1 reverse transcriptase L289K mutation (extrapolated to zero concentration) Sample: Gag-Pol polyprotein (HIV-1 reverse transcriptase C38V/C280S, L289K truncated to 1 - 556) monomer, 65 kDa Human immunodeficiency virus … protein Buffer: 50 mM Bis-Tris pH 6.8, 100 mM NaCl, 1% glycerol, 0.02% w/v NaN3, pH: 6.8 Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Jun 20 RgGuinier 3.2 nm Dmax 11.1 nm VolumePorod 90 nm3