Open-Bundle Structure as the Unfolding Intermediate of Cytochrome c′ Revealed by Small Angle Neutron Scattering

Yamaguchi T, Akao K, Koutsioubas A, Frielinghaus H, Kohzuma T
Biomolecules 12(1):95 (2022 Jan 07)

doi: 10.3390/biom12010095

Submitted to SASBDB: 2021 Dec 9 Published in SASBDB: 2022 Jan 7

SASDN52 – SANS data from cytochrome c' from Alcaligenes xylosoxidans at pD = 1.7 Sample: Cytochrome c' monomer, 14 kDa Achromobacter xylosoxidans protein Buffer: Phosphate Buffer pD 1.7, pH: 1.7 Experiment: SANS data collected at KWS1, FRM2 on 2017 Aug 12 RgGuinier 2.3 nm Dmax 8.6 nm VolumePorod 13 nm3

SASDN62 – SANS data from cytochrome c' from Alcaligenes xylosoxidans at pD = 6.4 Sample: Cytochrome c' dimer, 27 kDa Alcaligenes protein Buffer: Phosphate Buffer pD 6.4, pH: 6.4 Experiment: SANS data collected at KWS1, FRM2 on 2017 Aug 12 RgGuinier 1.8 nm Dmax 5.5 nm VolumePorod 11 nm3

SASDN72 – SANS data from cytochrome c' from Alcaligenes xylosoxidans at pD = 9.6 Sample: Cytochrome c' dimer, 27 kDa Alcaligenes protein Buffer: Phosphate Buffer pD 9.6, pH: 9.6 Experiment: SANS data collected at KWS1, FRM2 on 2017 Aug 12 RgGuinier 1.9 nm Dmax 5.3 nm VolumePorod 10 nm3

SASDN82 – SANS data from cytochrome c' from Alcaligenes xylosoxidans at pD = 13 Sample: Cytochrome c' monomer, 14 kDa Achromobacter xylosoxidans protein Buffer: Phosphate Buffer pD 13, pH: 13 Experiment: SANS data collected at KWS1, FRM2 on 2017 Aug 12 RgGuinier 4.8 nm Dmax 9.0 nm VolumePorod 20 nm3