Overall structure of fully assembled cyanobacterial KaiABC circadian clock complex by an integrated experimental-computational approach.

Yunoki Y, Matsumoto A, Morishima K, Martel A, Porcar L, Sato N, Yogo R, Tominaga T, Inoue R, Yagi-Utsumi M, Okuda A, Shimizu M, Urade R, Terauchi K, Kono H, Yagi H, Kato K, Sugiyama M
Commun Biol 5(1):184 (2022 Mar 10)

PMID: 35273347 doi: 10.1038/s42003-022-03143-z

Submitted to SASBDB: 2021 Dec 15 Published in SASBDB: 2022 Feb 21

SASDNJ2 – Circadian clock KaiA-KaiB-KaiC ternary protein complex measured by SEC-SAXS Sample: Circadian clock protein KaiA dodecamer, 393 kDa Synechococcus elongatus (strain … protein Circadian clock protein KaiB hexamer, 71 kDa Synechococcus elongatus (strain … protein Circadian clock protein kinase KaiC (S431D mutant) hexamer, 357 kDa Synechococcus elongatus (strain … protein Buffer: 50 mM sodium phosphate buffer, 150 mM NaCl, 5 mM MgCl2, 0.5 mM EDTA, 1 mM ATP, 1 mM DTT, 50 mM arginine, 50 mM glutamine, pH: 7.8 Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2017 Dec 8 RgGuinier 7.0 nm Dmax 24.5 nm VolumePorod 1650 nm3

SASDNK2 – Circadian clock hKaiA-75dKaiB-75dKaiC ternary protein complex measured by SEC-SANS in 100% D2O Sample: 75% deuterated Circadian clock protein KaiB hexamer, 71 kDa Synechococcus elongatus (strain … protein Circadian clock protein KaiA dodecamer, 393 kDa Synechococcus elongatus (strain … protein 75% deuterated Circadian clock protein kinase KaiC (S431D mutant) hexamer, 357 kDa Synechococcus elongatus (strain … protein Buffer: 50 mM sodium phosphate buffer, 150 mm NaCl, 5 mM MgCl2, 0.5 mM EDTA, 1 mM ATP, 1 mM DTT, 50 mM arginine, 50 mM glutamine, in 100% D2O, pH: 7.8 Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2018 Sep 19 RgGuinier 7.8 nm Dmax 25.6 nm VolumePorod 1620 nm3