Self-Assembling Lectin Nano-Block Oligomers Enhance Binding Avidity to Glycans

Irumagawa S, Hiemori K, Saito S, Tateno H, Arai R
International Journal of Molecular Sciences 23(2):676 (2022 Jan 08)

doi: 10.3390/ijms23020676

Submitted to SASBDB: 2021 Dec 28 Published in SASBDB: 2022 Jan 23

SASDNC3 – Lectin nano-block dimer of WA20-SL-ACG Sample: Lectin nano-block WA20-SL-ACG dimer, 60 kDa protein Buffer: 20 mM HEPES, 150 mM NaCl, 5% glycerol,, pH: 7.5 Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2020 Jun 20 RgGuinier 3.1 nm Dmax 14.9 nm VolumePorod 82 nm3

SASDND3 – Lectin nano-block tetramer of WA20-SL-ACG Sample: Lectin nano-block WA20-SL-ACG tetramer, 121 kDa protein Buffer: 20 mM HEPES, 150 mM NaCl, 5% glycerol,, pH: 7.5 Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2020 Jun 20 RgGuinier 4.7 nm Dmax 18.6 nm VolumePorod 183 nm3

SASDNE3 – Lectin nano-block hexamer of WA20-SL-ACG Sample: Lectin nano-block WA20-SL-ACG hexamer, 181 kDa protein Buffer: 20 mM HEPES, 150 mM NaCl, 5% glycerol,, pH: 7.5 Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2020 Jun 20 RgGuinier 5.4 nm Dmax 24.2 nm VolumePorod 313 nm3

SASDNF3 – Lectin nano-block dimer of WA20-H-ACG Sample: Lectin nano-block WA20-H-ACG dimer, 59 kDa protein Buffer: 20 mM HEPES, 150 mM NaCl, 5% glycerol,, pH: 7.5 Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2020 Oct 31 RgGuinier 3.9 nm Dmax 18.1 nm VolumePorod 85 nm3

SASDNG3 – Lectin nano-block dimer of WA20-ΔN3ACG Sample: Lectin nano-block WA20-ΔN3ACG dimer, 58 kDa protein Buffer: 20 mM HEPES, 150 mM NaCl, 5% glycerol,, pH: 7.5 Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2020 Oct 31 RgGuinier 4.1 nm Dmax 21.6 nm VolumePorod 87 nm3