Elucidation of critical pH-dependent structural changes in Botulinum Neurotoxin E.

Lalaurie CJ, Splevins A, Barata TS, Bunting KA, Higazi DR, Zloh M, Spiteri VA, Perkins SJ, Dalby PA
J Struct Biol :107876 (2022 Jun 20)
PMID: 35738335
doi: 10.1016/j.jsb.2022.107876 		Submitted to SASBDB: 2022 Feb 18
Published in SASBDB:

SASDNV5 – Botulinum Toxin E at acidic pH

Botulinum Toxin Serotype E, endonegative experimental SAS data
GROMACS model
Sample: Botulinum Toxin Serotype E, endonegative monomer, 144 kDa Clostridium botulinum protein
Buffer: 10 mM sodium acetate (20 mM ionic strength), pH: 4
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Oct 6
RgGuinier 4.3 nm
Dmax 15.2 nm
VolumePorod 240 nm3