Modulation of the substrate specificity of the kinase PDK1 by distinct conformations of the full-length protein

Sacerdoti M, Gross L, Riley A, Zehnder K, Ghode A, Klinke S, Anand G, Paris K, Winkel A, Herbrand A, Godage H, Cozier G, Süß E, Schulze J, Pastor-Flores D, Bollini M, Cappellari M, Svergun D, Gräwert M, Aramendia P, Leroux A, Potter B, Camacho C, Biondi R
Science Signaling 16(789) (2023 Jun 13)

doi: 10.1126/scisignal.add3184

Submitted to SASBDB: 2022 Feb 8 Published in SASBDB: 2023 Jun 14

SASDNJ5 – Full length 3-phosphoinositide-dependent protein kinase (PDK1) Sample: 3-phosphoinositide-dependent protein kinase 1 monomer, 65 kDa Homo sapiens protein Buffer: 20 mM Tris-HCl pH 7.4, 250 mM NaCl, 1 mM DTT, pH: 7.4 Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 26 RgGuinier 3.5 nm Dmax 11.2 nm VolumePorod 107 nm3

SASDNK5 – Full length 3-phosphoinositide-dependent protein kinase (PDK1) in the presence of HYG8 (2-O-benzoyl-Ins(1,3,4,5,6)P5) Sample: 3-phosphoinositide-dependent protein kinase 1 monomer, 65 kDa Homo sapiens protein 2-O-benzoyl-Ins(1,3,4,5,6)P5 monomer, 1 kDa synthetic construct Buffer: 20 mM Tris-HCl pH 7.4, 250 mM NaCl, 1 mM DTT, 1 μM HYG8, pH: 7.4 Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 26 RgGuinier 3.5 nm Dmax 11.5 nm VolumePorod 98 nm3

SASDNL5 – Double mutant catalytic domain of 3-phosphoinositide-dependent protein kinase 1 (PDK1 50–359; Y188G Q292A) Sample: 3-phosphoinositide-dependent protein kinase 1 (Y188G Q292A) monomer, 35 kDa Homo sapiens protein Buffer: 20 mM Tris-HCl pH 7.4, 250 mM NaCl, 1 mM DTT, pH: 7.4 Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 26 RgGuinier 2.4 nm Dmax 7.0 nm VolumePorod 57 nm3