Allosteric communication between DNA-binding and light-responsive domains of diatom class I aureochromes.

Banerjee A, Herman E, Serif M, Maestre-Reyna M, Hepp S, Pokorny R, Kroth PG, Essen LO, Kottke T
Nucleic Acids Res 44(12):5957-70 (2016 Jul 8)
PMID: 27179025
doi: 10.1093/nar/gkw420 		Submitted to SASBDB: 2016 Feb 14
Published in SASBDB:

SASDBX3 – Aureobox dsDNA

Aureobox dsDNA experimental SAS data
DAMFILT model
Sample: Aureobox dsDNA monomer, 13 kDa synthetic construct DNA
Buffer: 50 mM Tris 50 mM boric acid 1 mM EDTA, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2014 Nov 6
RgGuinier 2.0 nm
Dmax 8.6 nm
VolumePorod 18 nm3

SASDBY3 – Aureochrome 1a bZIP-LOV module: PtAUREO1a bZIP-LOV (Light oxygen voltage) module (light state-TBE)

Aureochrome 1a bZIP-LOV module experimental SAS data
DAMFILT model
Sample: Aureochrome 1a bZIP-LOV module dimer, 57 kDa Phaeodactylum tricornutum protein
Buffer: 50 mM Tris 50 mM boric acid 1 mM EDTA, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2014 Nov 6
RgGuinier 3.4 nm
Dmax 12.6 nm
VolumePorod 115 nm3

SASDBZ3 – Aureochrome 1a bZIP-LOV module: PtAUREO1a bZIP-LOV (Light oxygen voltage)/DNA complex (light state)

Aureobox dsDNAAureochrome 1a bZIP-LOV module experimental SAS data
DAMFILT model
Sample: Aureobox dsDNA monomer, 13 kDa synthetic construct DNA
Aureochrome 1a bZIP-LOV module dimer, 57 kDa Phaeodactylum tricornutum protein
Buffer: 50 mM Tris 50 mM boric acid 1 mM EDTA, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2014 Nov 6
RgGuinier 4.5 nm
Dmax 16.7 nm
VolumePorod 97 nm3

SASDB24 – Aureochrome 1a bZIP-LOV module: PtAUREO1a bZIP-LOV (Light oxygen voltage) module (light state, Tris)

Aureochrome 1a bZIP-LOV module experimental SAS data
Aureochrome 1a bZIP-LOV module Kratky plot
Sample: Aureochrome 1a bZIP-LOV module dimer, 57 kDa Phaeodactylum tricornutum protein
Buffer: 10 mM Tris 300 mM NaCl, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2014 Nov 6
RgGuinier 3.9 nm
Dmax 12.5 nm
VolumePorod 121 nm3

SASDB34 – Aureochrome 1a bZIP-LOV module: PtAUREO1a bZIP-LOV (Light oxygen voltage) module (dark state, Tris)

Aureochrome 1a bZIP-LOV module experimental SAS data
Aureochrome 1a bZIP-LOV module Kratky plot
Sample: Aureochrome 1a bZIP-LOV module dimer, 57 kDa Phaeodactylum tricornutum protein
Buffer: 10 mM Tris 300 mM NaCl, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2014 Nov 6
RgGuinier 3.8 nm
Dmax 12.5 nm
VolumePorod 117 nm3