Recombinant AcnB, NrdR and RibD of Acinetobacter baumannii and their potential interaction with DNA adenine methyltransferase AamA.

Weber K, Doellinger J, Jeffries CM, Wilharm G
Protein Expr Purif :106134 (2022 Jul 1)

PMID: 35787944 doi: 10.1016/j.pep.2022.106134

Submitted to SASBDB: 2022 May 14 Published in SASBDB: 2022 Jun 14

SASDNC5 – Acinetobacter baumannii riboflavin biosynthesis protein RibD Sample: Riboflavin biosynthesis protein RibD dimer, 80 kDa Acinetobacter baumannii protein Buffer: 150 mM NaCl, 10 mM Tris, 1 mM DTT, 5% v/v glycerol, pH: 7.4 Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Dec 3 RgGuinier 3.7 nm Dmax 12.0 nm VolumePorod 115 nm3

SASDND5 – Acinetobacter baumannii aconitate hydratase B, monomer fraction Sample: Aconitate hydratase B monomer, 96 kDa Acinetobacter baumannii protein Buffer: 100 mM NaCl, 20 mM Tris, 1 mM DTT, 3% v/v glycerol, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Dec 3 RgGuinier 3.4 nm Dmax 12.5 nm VolumePorod 150 nm3

SASDNE5 – Acinetobacter baumannii aconitate hydratase B, monomer/dimer mixed fraction Sample: Aconitate hydratase B, 96 kDa Acinetobacter baumannii protein Buffer: 100 mM NaCl, 20 mM Tris, 1 mM DTT, 3% v/v glycerol, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Dec 3 RgGuinier 4.0 nm Dmax 14.0 nm VolumePorod 230 nm3

SASDNF5 – Acinetobacter baumannii transcriptional repressor NrdR (likely filamentous assembly) Sample: Transcriptional repressor NrdR, 19 kDa Acinetobacter baumannii (strain … protein Buffer: 100 mM NaCl, 20 mM Tris, 1 mM DTT, 3% v/v glycerol, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Dec 3 RgGuinier 19.9 nm Dmax 92.6 nm