Using stable MutS dimers and tetramers to quantitatively analyze DNA mismatch recognition and sliding clamp formation.

Groothuizen FS, Fish A, Petoukhov MV, Reumer A, Manelyte L, Winterwerp HH, Marinus MG, Lebbink JH, Svergun DI, Friedhoff P, Sixma TK
Nucleic Acids Res 41(17):8166-81 (2013 Sep)

PMID: 23821665 doi: 10.1093/nar/gkt582

Published in SASBDB: 2015 Jul 7

SASDAN3 – MutS dimer Sample: DNA mismatch repair protein MutS dimer, 191 kDa Escherichia coli protein Buffer: 50 mM HEPES 50 mM KCl, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Feb 28 RgGuinier 4.7 nm Dmax 15.5 nm VolumePorod 307 nm3

SASDAQ3 – MutS tetramer Sample: DNA mismatch repair protein MutS tetramer, 381 kDa Escherichia coli protein Buffer: 50 mM HEPES 50 mM KCl, pH: 7.5 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2011 May 12 RgGuinier 7.8 nm Dmax 28.0 nm VolumePorod 700 nm3

SASDAX3 – MutS tetramer Sample: DNA mismatch repair protein MutS tetramer, 381 kDa Escherichia coli protein Buffer: 50 mM HEPES 50 mM KCl, pH: 7.5 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2011 May 12 RgGuinier 8.5 nm Dmax 29.0 nm VolumePorod 750 nm3

SASDAY3 – MutS tetramer Sample: DNA mismatch repair protein MutS tetramer, 381 kDa Escherichia coli protein Buffer: 50 mM HEPES 50 mM KCl, pH: 7.5 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2011 May 12 RgGuinier 8.3 nm Dmax 29.0 nm VolumePorod 720 nm3

SASDAZ3 – MutS tetramer Sample: DNA mismatch repair protein MutS tetramer, 381 kDa Escherichia coli protein Buffer: 50 mM HEPES 50 mM KCl, pH: 7.5 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2011 May 12 RgGuinier 8.0 nm

SASDA24 – MutS tetramer Sample: DNA mismatch repair protein MutS tetramer, 381 kDa Escherichia coli protein Buffer: 50 mM HEPES 50 mM KCl, pH: 7.5 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2011 May 12 RgGuinier 7.8 nm Dmax 27.0 nm