Conformational buffering underlies functional selection in intrinsically disordered protein regions.

González-Foutel NS, Glavina J, Borcherds WM, Safranchik M, Barrera-Vilarmau S, Sagar A, Estaña A, Barozet A, Garrone NA, Fernandez-Ballester G, Blanes-Mira C, Sánchez IE, de Prat-Gay G, Cortés J, Bernadó P, Pappu RV, Holehouse AS, Daughdrill GW, Chemes LB
Nat Struct Mol Biol (2022 Aug 10)

PMID: 35948766 doi: 10.1038/s41594-022-00811-w

Submitted to SASBDB: 2022 Mar 7 Published in SASBDB: 2022 Aug 12

SASDNK6 – Retinoblastoma protein at 1 mg/ml Sample: Retinoblastoma-associated protein monomer, 41 kDa Homo sapiens protein Buffer: 20 mM sodium phosphate pH 7.0, 200 mM NaCl, 1mM DTT, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Jul 14 RgGuinier 2.4 nm Dmax 7.4 nm VolumePorod 66 nm3

SASDNL6 – Retinoblastoma protein at 2 mg/ml Sample: Retinoblastoma-associated protein monomer, 41 kDa Homo sapiens protein Buffer: 20 mM sodium phosphate pH 7.0, 200 mM NaCl, 1mM DTT, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Jul 14 RgGuinier 2.5 nm Dmax 7.9 nm VolumePorod 64 nm3

SASDNM6 – Retinoblastoma protein at 4 mg/ml Sample: Retinoblastoma-associated protein monomer, 41 kDa Homo sapiens protein Buffer: 20 mM sodium phosphate pH 7.0, 200 mM NaCl, 1mM DTT, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Jul 14 RgGuinier 2.6 nm Dmax 10.0 nm VolumePorod 68 nm3

SASDNN6 – Early E1A protein at 4.2 mg/ml Sample: Early E1A protein monomer, 13 kDa Human adenovirus C … protein Buffer: 20 mM sodium phosphate pH 7.0, 200 mM NaCl, 1mM DTT, pH: 7 Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 24 RgGuinier 4.0 nm

SASDNP6 – Early E1A protein at 5.6 mg/ml Sample: Early E1A protein monomer, 13 kDa Human adenovirus C … protein Buffer: 20 mM sodium phosphate pH 7.0, 200 mM NaCl, 1mM DTT, pH: 7 Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 24 RgGuinier 4.2 nm

SASDNQ6 – Early E1A protein at 7.0 mg/ml Sample: Early E1A protein monomer, 13 kDa Human adenovirus C … protein Buffer: 20 mM sodium phosphate pH 7.0, 200 mM NaCl, 1mM DTT, pH: 7 Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 24 RgGuinier 4.1 nm

SASDNR6 – Retinoblastoma protein/Early E1A protein (RB-E1A) complex at 0.7 mg/ml Sample: Retinoblastoma-associated protein monomer, 41 kDa Homo sapiens protein Early E1A protein monomer, 13 kDa Human adenovirus C … protein Buffer: 20 mM sodium phosphate pH 7.0, 200 mM NaCl, 1mM DTT, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Jul 14 RgGuinier 2.9 nm Dmax 10.0 nm VolumePorod 80 nm3

SASDNS6 – Retinoblastoma protein/Early E1A protein (RB-E1A) complex at 1.4 mg/ml Sample: Retinoblastoma-associated protein monomer, 41 kDa Homo sapiens protein Early E1A protein monomer, 13 kDa Human adenovirus C … protein Buffer: 20 mM sodium phosphate pH 7.0, 200 mM NaCl, 1mM DTT, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Jul 14 RgGuinier 3.0 nm Dmax 12.0 nm VolumePorod 84 nm3

SASDNT6 – Retinoblastoma protein/Early E1A protein (RB-E1A) complex at 2.7 mg/ml Sample: Retinoblastoma-associated protein monomer, 41 kDa Homo sapiens protein Early E1A protein monomer, 13 kDa Human adenovirus C … protein Buffer: 20 mM sodium phosphate pH 7.0, 200 mM NaCl, 1mM DTT, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Jul 14 RgGuinier 3.3 nm Dmax 17.0 nm VolumePorod 93 nm3

SASDNU6 – Retinoblastoma protein/Early E1A protein (RB-E1A) complex - merged data from concentration series measurements Sample: Retinoblastoma-associated protein monomer, 41 kDa Homo sapiens protein Early E1A protein monomer, 13 kDa Human adenovirus C … protein Buffer: 20 mM sodium phosphate pH 7.0, 200 mM NaCl, 1mM DTT, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Jul 14 RgGuinier 3.0 nm Dmax 12.0 nm VolumePorod 84 nm3

SASDNV6 – Early E1A protein, SEC-SAXS data Sample: Early E1A protein monomer, 13 kDa Human adenovirus C … protein Buffer: 20 mM sodium phosphate pH 7.0, 200 mM NaCl, 1mM DTT, pH: 7 Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 24 RgGuinier 3.6 nm Dmax 17.5 nm VolumePorod 45 nm3