The Conformation of the N-Terminal Tails of Deinococcus grandis Dps Is Modulated by the Ionic Strength

Guerra J, Blanchet C, Vieira B, Almeida A, Waerenborgh J, Jones N, Hoffmann S, Tavares P, Pereira A
International Journal of Molecular Sciences 23(9):4871 (2022 Apr 28)

doi: 10.3390/ijms23094871

Submitted to SASBDB: 2022 Mar 25 Published in SASBDB: 2022 May 2

SASDNY7 – DNA-binding protein from starved cells: DgrDpsWT in 50 mM MOPS, 50 mM NaCl pH 7.0 Sample: DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein Buffer: 50 mM MOPS-NaOH, 50 mM NaCl, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Oct 22 RgGuinier 4.4 nm Dmax 16.1 nm VolumePorod 409 nm3

SASDNZ7 – DNA-binding protein from starved cells: DgrDpsWT in 50 mM MOPS, 80 mM NaCl pH 7.0 Sample: DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein Buffer: 50 mM MOPS-NaOH, 80 mM NaCl, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Oct 22 RgGuinier 4.5 nm Dmax 17.1 nm VolumePorod 475 nm3

SASDN28 – DNA-binding protein from starved cells: DgrDpsWT in 50 mM MOPS, 230 mM NaCl pH 7.0 Sample: DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein Buffer: 50 mM MOPS-NaOH, 230 mM NaCl, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Oct 22 RgGuinier 4.5 nm Dmax 20.6 nm VolumePorod 438 nm3

SASDN38 – DNA-binding protein from starved cells: DgrDpsWT in 50 mM MOPS, 480 mM NaCl pH 7.0 Sample: DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein Buffer: 50 mM MOPS-NaOH, 480 mM NaCl, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Oct 22 RgGuinier 4.8 nm Dmax 20.5 nm VolumePorod 430 nm3

SASDN48 – DNA-binding protein from starved cells (tailless): DgrDps∆N in 50 mM MOPS, 50 mM NaCl pH 7.0 Sample: DNA protection during starvation, DPS-∆N (Ferritin superfamily) dodecamer, 218 kDa Deinococcus grandis protein Buffer: 50 mM MOPS-NaOH, 50 mM NaCl, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Oct 22 RgGuinier 3.8 nm Dmax 9.3 nm VolumePorod 290 nm3

SASDN58 – DNA-binding protein from starved cells (tailless): DgrDps∆N in 50 mM MOPS, 230 mM NaCl pH 7.0 Sample: DNA protection during starvation, DPS-∆N (Ferritin superfamily) dodecamer, 218 kDa Deinococcus grandis protein Buffer: 50 mM MOPS-NaOH, 230 mM NaCl, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Oct 22 RgGuinier 3.8 nm Dmax 8.8 nm VolumePorod 291 nm3