Structural analysis and functional evaluation of the disordered ß–hexosyltransferase region from Hamamotoa (Sporobolomyces) singularis

Dagher S, Vaishnav A, Stanley C, Meilleur F, Edwards B, Bruno-Bárcena J
Frontiers in Bioengineering and Biotechnology 11 (2023 Dec 14)

doi: 10.3389/fbioe.2023.1291245 		Submitted to SASBDB: 2022 Mar 14
Published in SASBDB:

SASDN57 – beta-hexosyl transferase (23-594), 1 mg/mL

Beta-galactosidase-like enzyme experimental SAS data
Beta-galactosidase-like enzyme Kratky plot
Sample: Beta-galactosidase-like enzyme dimer, 129 kDa Hamamotoa singularis protein
Buffer: 5 mM sodium phosphate, pH: 5
Experiment: SAXS data collected at Rigaku BioSAXS-2000, Oak Ridge National Laboratory on 2018 Jun 25
RgGuinier 3.9 nm
Dmax 12.4 nm
VolumePorod 238 nm3

SASDN67 – beta-hexosyl transferase (23-594), 4 mg/mL

Beta-galactosidase-like enzyme experimental SAS data
Beta-galactosidase-like enzyme Kratky plot
Sample: Beta-galactosidase-like enzyme dimer, 129 kDa Hamamotoa singularis protein
Buffer: 5 mM sodium phosphate, pH: 5
Experiment: SAXS data collected at Rigaku BioSAXS-2000, Oak Ridge National Laboratory on 2018 Jun 25
RgGuinier 3.7 nm
Dmax 12.5 nm
VolumePorod 236 nm3