The plant nucleoplasmin AtFKBP43 needs its extended arms for histone interaction.

Singh AK, Saharan K, Baral S, Vasudevan D
Biochim Biophys Acta Gene Regul Mech 1865(7):194872 (2022 Oct)

PMID: 36058470 doi: 10.1016/j.bbagrm.2022.194872

Submitted to SASBDB: 2022 Mar 25 Published in SASBDB: 2023 Mar 20

SASDNN3 – Peptidyl-prolyl cis-trans isomerase FKBP43, nucleoplasmin domain (amino acids 1-96) Sample: Peptidyl-prolyl cis-trans isomerase FKBP43 pentamer, 58 kDa Arabidopsis thaliana protein Buffer: 20 mM Tris, 300 mM NaCl, 1 mM β-mercaptoethanol, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2018 Mar 16 RgGuinier 2.5 nm Dmax 7.3 nm VolumePorod 141 nm3

SASDNQ7 – Peptidyl-prolyl cis-trans isomerase AtFKBP43 (1-136) Sample: Peptidyl-prolyl cis-trans isomerase FKBP43 pentamer, 81 kDa Arabidopsis thaliana protein Buffer: 20 mM Tris, 300 mM NaCl, 1 mM β-mercaptoethanol, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2021 Apr 28 RgGuinier 3.6 nm Dmax 12.4 nm VolumePorod 164 nm3

SASDNT7 – Peptidyl-prolyl cis-trans isomerase AtFKBP43 (1-164) Sample: Peptidyl-prolyl cis-trans isomerase FKBP43 pentamer, 96 kDa Arabidopsis thaliana protein Buffer: 20 mM Tris, 300 mM NaCl, 1 mM β-mercaptoethanol, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2021 Apr 28 RgGuinier 4.0 nm Dmax 13.9 nm VolumePorod 180 nm3

SASDNR7 – Peptidyl-prolyl cis-trans isomerase AtFKBP43 (1-136) -H2A/H2B histone oligomer complex Sample: Peptidyl-prolyl cis-trans isomerase FKBP43 pentamer, 81 kDa Arabidopsis thaliana protein Histone H2A type 1-B/E monomer, 14 kDa Homo sapiens protein Histone H2B type 1-C/E/F/G/I monomer, 14 kDa Homo sapiens protein Buffer: 20 mM Tris, 300 mM NaCl, 1 mM β-mercaptoethanol, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2021 Apr 28 RgGuinier 4.1 nm Dmax 13.9 nm VolumePorod 194 nm3

SASDNS7 – Peptidyl-prolyl cis-trans isomerase AtFKBP43 (1-136) -H3/H4 histone oligomer complex Sample: Peptidyl-prolyl cis-trans isomerase FKBP43 pentamer, 81 kDa Arabidopsis thaliana protein Histone H3.1 dimer, 31 kDa Homo sapiens protein Histone H4 dimer, 23 kDa Homo sapiens protein Buffer: 20 mM Tris, 300 mM NaCl, 1 mM β-mercaptoethanol, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2021 Apr 28 RgGuinier 4.4 nm Dmax 14.1 nm VolumePorod 226 nm3